ANALYSIS OF SIMULATED DIFFUSION AND ADSORPTION WITH AN ANALYTICAL MODEL FOR FLUORESCENCE CORRELATION SPECTROSCOPY

Abstract

Fluorescence correlation spectroscopy (FCS) is a powerful tool to predict molecular diffusivity in femtoliter observation volume. The applicable analytical model in 3-D for porous chromatographic environment has been introduced to evaluate in this contribution. Regarding chromatographic environment, it is important to understand and characterize both diffusion and adsorption-desorption kinetics at interfacial area between mobile and stationary phases. In this contribution, a demonstration of a newly designed analytical model for FCS was presented that can predict simulated diffusion and adsorption-desorption kinetics in 3-D matrix. This work was motivated by a previous 2-D analytical solution study. The range of desorption rate (800–1000 1/s) was found to be where the analytical model can predict the molecular dynamics most accurately and precisely.