Determination of the antagonistic efficacy of silver nanoparticles against two major strains of Mycobacterium tuberculosis

Soumok Sadhu; Tamoghna Karmakar; Anindya Gopal Chatterjee; Uma Kumari; Purav Mondal; Shrubawati Sarka; Taniya Sur; Sambit Tarafdar

doi:10.52756/ijerr.2022.v29.007

Authors

Soumok Sadhu Demonstrator in MVLS Department, University of Glasgow, Scotland https://orcid.org/0000-0003-4186-254X
Tamoghna Karmakar Department of Biochemistry, University of Calcutta, West Bengal, India
Anindya Gopal Chatterjee Department of Zoology, Raja Peary Mohan College, Kolkata, West Bengal, India
Uma Kumari Department of Biotechnology, Radha Govind University, Lalki Ghati, Ramgarh, Jharkhand, India
Purav Mondal Department of Biochemistry, University of Calcutta, West Bengal, India
Shrubawati Sarka Department of Zoology, Derozio Memorial College, West Bengal, India
Taniya Sur School of Biotechnology Kalinga Institute of Industrial Technology University, Bhubaneshwar, Orissa, India
Sambit Tarafdar Amity Institute of Virology and Immunology, Amity University, Noida, India

DOI:

https://doi.org/10.52756/ijerr.2022.v29.007

Keywords:

Multi-drug resistant, Mycobacterium,, Nanoparticles, Silver, Tuberculosis

Abstract

Tuberculosis (TB) is considered one of the most prominent diseases across the globe. This present study aims to inspect the impact of silver nanoparticles (AgNP) against Mycobacterium tuberculosis, which is the causative vector of TB. The efficacy of the AgNP was conducted based on the minimum inhibitory concentration (MIC) of the AgNPs through microplate Alamar blue assay. The preparation of the AgNPs involved chemical synthesis. The state and the size of the AgNPs were determined and confirmed by using ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray diffraction (XRD) spectroscopy, and Transmission electron microscopy (TEM). This study evaluated two strains: Mycobacterium tuberculosis H37Rv and Mycobacterium bovis. In addition, another multiple drug-resistant Mycobacterium tuberculosis strain was also considered in this study, along with the clinically obtained isolates from Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis bovis. The synthesized nanoparticles were found to be tetrahedral in shape with an average particle size of 45±3 nanometre (nm). The obtained results indicated that the proliferation of all the strains (two reference strains and one MDR strain) was resisted by the action of the synthesized AgNPs. The MIC of the MDR strain was noted within the range of 2-12 µg/ml, whereas the MIC for the Mycobacterium tuberculosis H37Rv and Mycobacterium bovis was noted in the range of 2-14 and 3-30 µg/ml, respectively. Accordingly, this study proposed a novel approach to combat tuberculosis, which is considered a global threat to humankind, indicating the present study's novelty.

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