Fenugreek Seed Extract Mitigates MSG-Induced Uterine Dysfunction in Rats through Enhanced Antioxidative Defense Mechanisms

Mukti Mondal; Kaushik Sarkar; Goutam Paul

doi:10.52756/ijerr.2024.v37spl.012

Mukti Mondal Department of Physiology, Krishnath College, Berhampore, West Bengal, India https://orcid.org/0009-0009-9578-3258
Kaushik Sarkar Department of Physiology, Krishnagar Government College, Krishnagar, West Bengal, India https://orcid.org/0009-0002-3746-0492
Goutam Paul Department of Physiology, University of Kalyani, Kalyani, West Bengal, India

DOI: https://doi.org/10.52756/ijerr.2024.v37spl.012

Keywords: Monosodium glutamate, Fenugreek seed extracts, histo-architectural changes, antioxidative defense

Abstract

To assess the mitigating effectiveness of fenugreek seed extract in MSG-induced suppression of uterine function in rats, the effects of MSG in combination with fenugreek seed extract on the oxidative stress variables in the uterus and histo-architectural changes in the uterus have been studied and compared with the results obtained in the uterus in MSG-treated and control groups of rats. The study investigated the impact of MSG in conjunction with fenugreek seed extract on uterine function and oxidative stress. Female virgin albino rats were distributed into seven groups (Control, Treated I, Treated II, Treated III, Treated I+ Fenugreek seed extract, Treated II+ Fenugreek seed extract, Treated III+ Fenugreek seed extract) and subjected to thirty-day and forty-day of treatment via oral gavage. Despite no significant changes in mean body weight observed in MSG and fenugreek seed extract combination groups compared to the control, a notable counteraction was observed in the results obtained from rats exposed to MSG alone. This implies a possible protective function of fenugreek seed extract against oxidative stress in the uterus caused by MSG. Rats that received MSG in combination with fenugreek seed extract in their uterine tissue homogenate showed negligible changes in the activities of SOD, CAT, GR, GPx, GST, and MDA production when compared to the control group. Additionally, when comparing the MSG-exposed groups compare to control groups, a noteworthy decline in the activities of SOD, CAT, GR, and GPx and an increase in the activity of GST and MDA production were noted. When MSG was applied in conjunction with fenugreek seed extract for both treatment durations, no discernible histo-architectural alterations in the uterus were seen compared to the uterine tissues of the control groups of rats. Thus, it can be inferred from the findings that fenugreek seed extract significantly mitigated the oxidative stress and histo-architectural changes in rat uterine tissues caused by MSG.

References

Alao, O.A., Ashaolu, J.O., Ghazal, O.K., & Ukwenya, V.O.(2010). Histological and biochemical effects of MSG on the frontal lobe of adult wistar rats. International J. Health Biochem Sci., 6(4).

Atef, H., El-Morsi, D.A., El-Shafey, M., El-Sherbiny, M., El-Kattawy, H.A., Fahmy, E.K., & Saeed, A.A.A. (2019). Monosodium Glutamate Induced Hepatotoxicity and Oxidative Stress: Pathophysiological, Biochemical and Electron Microscopic Study. The Medical Journal of Cairo University, 87, 397-406. https://doi.org/10.21608/MJCU.2019.52361.

Bancroft, J.D., & Gamble, M. (2002). Theory and practice of histological techniques. Edinburgh Churchill Livingstone Pub. 5th Edn: pp. 172-175 and pp. 593-620.

Bayram, H.M., Akgoz, H.F., Kizildemir, O., & Ozturkean, A. (2023). Monosodium glutamate: review on preclinical and clinical reports. Biointerface Research in Applied Chemistry, 13(2). https://doi.org/10.33263/BRIAC132.149

Collison, K.S., Makhoul, N.J., Zaidi, M.Z., Al-Rabiah, R., Inglis, A., Andres, B.L., Ubungen, R., Shoukri, M., & Al-Mohanna, F.A. (2012). Interactive Effects of Neonatal Exposure to Monosodium Glutamate and Aspartame on Glucose Homeostasis. Nutrition and Metabolism (Lond), 9(1), 58. https://doi.org/10.1186/1743-7075-9-58

Das, R.S., & Ghosh, S.K. (2010). Long Term Effects of Monosodium Glutamate on Spermatogenesis Following Neonatal Exposure in Albino Mice--A Histological Study. Nepal Medical College Journal, 12(3), 149-153.

Das, R.S., & Ghosh, S.K. (2011). Long-Term Effects in Ovaries of the Adult Mice Following Exposure to Monosodium Glutamate during Neonatal Life-A Histological Study. Nepal Medical College Journal, 13(2), 77-83.

Devasagayam, T.P.A., & Tarachand, U. (1987). Decreased lipid peroxidation in the rat kidney during gestation. Biochem Biophys Res Commun, 56, 836-842. https://doi.org/10.1016/0006-291X(87)91297-6

Erb, J. (2006). A report on the toxic effects of the food additive monosodium glutamate. Joint FAO/WHO Expert Committee on food additives. pp. 400-460.

Eweka, A.O., Igbigbi, P.S., & Ucheya, R.E. (2011). Histochemical Studies of the Effects of Monosodium Glutamate on the Liver of Adult Wistar Rats. Annal of Medical and Health Sciences Research, 1(1), 21-29. https://doi.org/10.4314/abs.v9i1.66569

Evbuomwan, S.A., Omotosho, O.E., & Akinola, O.O. (2023). Monosodium glutamate: health risks, controversies and future perspectives. Agrociencia, 57(6), 25-54.

Frnestrom, J.D. (2018). Monosodium glutamate in the diet does not raise brain glutamate concentrations or disrupt brain functions. Ann. Nutr. Metabol., 73, 43-52.

Geha, R.S., Beiser, A., Ren, C., Patterson, R., Greenberger, P.A., Grammer, L.C., Ditto, A.M., Harris, K.E., Shaughnessy, M.A., Yarnold, P.R., Corren, J., & Saxon, A. (2000). Review of Alleged Reaction to Monosodium Glutamate and Outcome of a Multicenter Research. Article ID: 608765. https://doi.org/10.1093/jn/130.4.1058S

Habig, W.H., Pabst, M.J., & Jakoby, W.B. (1974). Glutathione-s-transferase: the first enzymatic step in mercapturic acid formation. J. Boil. Chem., 249,7130-7139. https://doi.org/10.1016/S0021-9258(19)42083-8

Husarova, V., & Ostatnikov, D. (2013). Monosodium Glutamate Toxic Effects and Their Implications for Human Intake: A Review. JMED Research, Article ID: 608765. https://doi.org/10.5171/2013.608765

JECFA, Joint FAO/WHO Expert Committee on Food Additives (JECFA). (1988). L-glutamic acid and its ammonium, calcium, monosodium and potassium salts. Toxicological evaluation of certain food additives and contaminants. pp. 97–161. New York Cambridge University Press.

Jinap, S., & Hajeb, P. (2010). Glutamate: its applications in food and contribution to health. Appetite, 55(1), 1-10. https://doi.org/10.1016/j.appet.2010.05.002

Kasozi, K.I., Namubiru, S., Kiconco, O., Kinyi, H.W., Ssempijja, F., Ezeonwumelu, J.O.C., Ninsiima, H.I., & Okpanachi, A.O. (2018). Low concentrations of monosodium glutamate (MSG) are safe in male Drosophila melanogaster. BMC Res. Notes, 11, 670. https://doi.org/10.1186/s13104-018-3775-x

Kaviarasan, S., Ramamurty, N., Gunasekaran, P., Varalakshmi, E., & Anuradha, C.V. (2006). Fenugreek (Trigonella foenumgraecum) seed prevents ethanolinduced toxicity and apoptosis in Chang liver cells. Alcohol Alcohol, 41, 267–273. https://doi.org/10.1093/alcalc/agl020

Kouzuki, M., Taniguchi, M., Suzuki, T., Nagano, M., Nakamura, S., Katsumata, Y., Matsumoto, H., & Urakami, K. (2019). Effect of monosodium L-glutamate (umami substance) on cognitive function in people with dementia. Eur. J. Clin. Nutr., 73, 266-275, https://doi.org/10.1038/s41430-018-0349-x.

Kumar U.S.U., Jothy,S.L., Gothai, S., Dharmaraj, S., Chen, Y., & Sasidharan, S. (2014). Standardization and Quality Evaluation of Cassia surattensis seed extract. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(5), 355-363.

Kumar, P. & Bhandari, U. (2013). Protective effect of Trigonella foenumgraecum Linn. on monosodium glutamate induced dyslipidemia and oxidative stress in rats. Indian J. Pharmacol, 45(2), 136–140. https://doi.org/10.4103/0253-7613.108288

Kwok, H.M. (1968). Chinese-restaurant syndrome. New England Journal of Medicine, 4, 796. https://doi.org/10.1056/NEJM196804042781419

Leung, A.Y., & Foster, S. (2003). Monosodium Glutamate: Encyclopedia of Common Natural Ingredients: Used in Food, Drugs, and Cosmetics (2nd Ed.). New York: Wiley. pp. 373-375.

Lima, C.B. (2013). Neonatal treatment with monosodium glutamate lastingly facilitates spreading depression in the rat cortex. Life Sciences, 93, 388-392. https://doi.org/10.1016/j.lfs.2013.07.009

Löliger, J. (2000). Function and importance of glutamate for savory foods. Journal of Nutrition, 130, 915S-920S. https://doi.org/10.1093/jn/130.4.915S

Lowry, O.H., Rosenbrough, N.J., Farr, A.L., & Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193(1), 265-275. https://doi.org/10.1016/S0021-9258(19)52451-6

Marklund, S., & Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47, 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

Miskowiak, B., & Partyka, M. (2000). Neonatal Treatment with Monosodium Glutamate (MSG): Structure of the TSH Immunoreactive Pituitary Cells. Histology and Histopathology, 5(2), 415-419.

Mohamed, P., Radwan, R., Mohamed, S.A., & Mohamed, S. (2021). Toxicity of monosodium glutamate on liver and body weight with the protective effect of tannic acid in adult male rats. Mansoura Journal of Forensic Medicine and Clinical Toxicology, 29, 23-32. https://doi.org/10.21608/MJFMCT.2021.58908.102

Mondal, M., Tarafder, P., Sarkar, K., Nath, P.P., & Paul, G. (2014). Monosodium Glutamate Induces Physiological Stress by Promoting Oxygen Deficiency, Cell Mediated Immunosuppression and Production of Cardiovascular Risk Metabolities in Rat. Int. J. Pharm. Sci. Rev. Res., 27(1), 328-331. https://doi.org/10.22376/ijpbs.2016.7.4.b799-804

Mondal, M., Sarkar, K., Nath, P.P., & Paul, G. (2016). Monosodium glutamate depresses the function of female reproductive system in rat by promoting oxidative stress induced changes in the structure of uterus. International Journal of Pharma and Bio Sciences,7(4), 799-804.

Morita, R., Ohta, M., Umeki, Y., Nanri, A., Tsuchihashi, T., & Hayabuchi, H. (2021). Effect of Monosodium Glutamate on Saltiness and Palatability Ratings of Low-Salt Solutions in Japanese Adults According to Their Early Salt Exposure or Salty Taste Preference. Nutrients, 13. https://doi.org/10.3390/nu13020577

Moskin, J. (2008). Yes, MSG, the Secret Behind the Savor. New York Times. Retrieved from http://www.nytimes.com.

Neely, E. (2013). Battle started with Krafts Food over Mac and Cheese ingredients. Examiner. http://www.examiner.com/article/battle-started-with-krafts-food-over-mac-and-cheese-ingredients.

Okediran, B.S., Olurotimi, A.E., Rahman, S.A., Michael, O.G., & Olukunle, J.O. (2014). Alteration in the lipid profile and liver enzymes in rats treated with monosodium glutamate. Sokoto Journal of Veterinary Sciences, 12(3), 42-46. https://doi.org/10.4314/sokjvs.v12i3.8

Pepino, M.Y., Finkbeiner, S., Beauchamp, G.K., & Mennella, J.A. (2010). Obese women have lower monosodium glutamate taste sensitivity and prefer higher concentrations than do normal‐weight women. Obesity, 18, 959-965. https://doi.org/10.1038/oby.2009.493.

Rotruck, J.T., Pope, A.L., Ganther, H.E., & Swanson, A.B. (1973). Selenium: Biochemical roles as a component of glutathione peroxidase. Science, 179, 588-590.

Samuel, A. (1969). The toxicity/safety of processed free acid (MSG). A study in suppression of information. Account Res., 6, 259-310. https://doi.org/10.1080/08989629908573933

Shah, N., Nariya, A., Pathan, A., Desai, P., Shah, J., Patel, A., Chettiar, S. S., & Jhala, D. (2019). Monosodium glutamate induced impairment in antioxidant defense system and genotoxicity in human neuronal cell line IMR-32. EurAsian J. Biosci., 13, 1121-1128.

Sinha, A.K. (1972). Colorimetric assay of catalase. Anal Biochem., 47, 389-394. https://doi.org/10.1016/0003-2697(72)90132-7

Staal, G.E.J., Visser, J., & Veeger, C. (1969). Purification and properties of glutathione reductase of human erythrocyte. Biochem. Biophys. Acta., 185, 39-48. https://doi.org/10.1016/0005-2744(69)90280-0

Walker, R., & Lupien, J.R. (2000). The safety evaluation of monosodium glutamate. Journal of Nutrition, 130(4S Suppl), 1049S–1052S. https://doi.org/10.1093/jn/130.4.1049S

Wijayasekara, K., & Wansapala, J. (2017). Uses, effects and properties of monosodium glutamate (MSG) on food and nutrition. Int. J. Fd. Sci. Nut., 2, 132-143.

Yamaguchi, S., & Ninomiya, K. (2000). Umami and Food Palatabily. The Journal of Nutrition, 30(4), 921-926. https://doi.org/10.1093/jn/130.4.921S

Zanfirescu, A., Ungurianu, A., Tsatsakis, A.M., Nitulescu, G.M., Kouretas, D., Eskoukis, A., Tsoukalas, D., Engin, A.B., Aschner, M. & Margina, D.(2019). A review of the alleged health hazards of monosodium glutamate. Compreh. Rev. Fd. Sci. Fd. Safety, 18, 1111-1134. https://doi.org/10.1111/1541-4337.12448

Zia, T., Hasnain, S.N., & Hasan, S.K. (2001). Evaluation of the oral hypoglycaemic effect of Trigonella foenumgraecum L. (methi) in normal mice. J. Ethnopharmacol, 75, 191–195. https://doi.org/10.1016/S0378-8741(01)00186-6

Yasodha, K., Lizha Mary, L., Surajit, P., & Satish, R. (2023). Exosomes from metastatic colon cancer cells drive the proliferation and migration of primary colon cancer through increased expression of cancer stem cell markers CD133 and DCLK1. Tissue and Cell, 84, 102163. https://doi.org/10.1016/J.TICE.2023.102163

Zhong, Y., Li, H., Li, P., Chen, Y., Zhang, M., Yuan, Z., Zhang, Y., Xu, Z., Luo, G., Fang, Y., & Li, X. (2021). Exosomes: A New Pathway for Cancer Drug Resistance. In Frontiers in Oncology, 11, 2021. https://doi.org/10.3389/fonc.2021.743556