Fabrication of microspheres and characterization of antimicrobial and anti-inflammatory activity isolated fraction from total alcoholic extract of Cassia Fistula (Linn.) in carrageenan-Type-IV induced inflammatory rats
DOI:
https://doi.org/10.52756/ijerr.2023.v32.021Keywords:
Microspheres, anti-inflammatory activity, Cassia fistula, carrageenan-Type IVAbstract
The Cassia fistula Linn is known as the golden shower tree. It’s also referred to as a "disease killer" in Ayurvedic medicine. The current study investigates the effect and estimate of antimicrobial and Anti-inflammatory activity guided fraction isolated from alcoholic extract of Cassia fistula (Linn.) root on carrageenan-Type IV induced Inflammatory rats for using Column fraction of alcoholic extract of root of Cassia fistula (Linn.) An in vivo carrageenan-induced paw edoema model was used to confirm further the anti-inflammatory effect of the fraction aloe-emodin and physcion-loaded microspheres. This study was designed first time for the isolation of alcoholic fraction of aloe emodin and Physcion by root extract and screening of pharmacological activities of Cassia fistula. Aloe-emodin and Physcion fraction demonstrated antibacterial and antifungal effectiveness against the studied pathogens in a dose-dependent manner, according to anti-microbial activity. 50 mg/ml concentration of Physcion-containing microspheres and aloe emodin's antibacterial efficacy against tested pathogens. The anti-inflammatory activity of Aloe emodin and Physcion was found in an equal manner. Both Microsphere samples were found capable of controlling the inflammation till 6 hours. According to the findings of this research, the chosen plant extracts have an immediate anti-inflammatory effect. There are three stages during which carrageenan-induced inflammation mediators are released.
References
Dama, G.Y., Tare, H. L., Gore, M. S., Shende, V. S., Deore, S. R., Khandagale, S. T., &Kandekar, A. E. (2011). Comparative cardiotonic activity of Helicteresisora with digoxin on isolated frog heart. International Journal of Preclinical and Pharmaceutical Research, 2(2), 81-86.
Dhadde, G. S., Mali, H. S., Raut, I. D., Nitalikar, M. M., & Bhutkar, M. A. (2021). A review on microspheres: Types, method of preparation, characterization and application. Asian Journal of Pharmacy and Technology, 11(2), 149-155. http://dx.doi.org/10.52711/2231-5713.2021.00025
Frent, O. D., Duda-Seiman, D. M., Vicas, L. G., Duteanu, N., Nemes, N. S., Pascu, B., ... & Marian, E. (2023). Study of the Influence of the Excipients Used for the Synthesis of Microspheres Loaded with Quercetin: Their Characterization and Antimicrobial Activity. Coatings, 13(8), 1376. https://doi.org/10.3390/coatings13081376
Hajare, P., Rai, V., Nipate, S., Balap, A., Pimple, B., Chumbhale, D., ... & Tare, H. (2023). Anti-arthritic potential of ethyl acetate fraction of Pinus roxburghii Sargent stem bark in Freund's complete adjuvant induced arthritis in Wistar rats. Multidisciplinary Science Journal, 5(4), 2023046-2023046. https://doi.org/10.31893/multiscience.2023046
He, S., & Mu, H. (2023). Microenvironmental pH Modification in Buccal/Sublingual Dosage Forms for Systemic Drug Delivery. Pharmaceutics, 15(2), 637. https://doi.org/10.3390/pharmaceutics15020637
Ju, Y., Zeng, H., Ye, X., Dai, M., Fang, B., & Liu, L. (2023). Zn2+ incorporated composite polysaccharide microspheres for sustained growth factor release and wound healing. Materials Today Bio, 100739. https://doi.org/10.1016/j.mtbio.2023.100739
Mane, V., Killedar, S., More, H., Salunkhe, S., & Tare, H. (2023). Development and Validation of a Novel Bioanalytical Method for Estimating Epigallocatechin 3 Gallate in Wistar Rat Plasma by RP-HPLC Employing Gradient Elution Techniques. Journal of Research in Pharmacy, 27(3). https://dx.doi.org/10.29228/jrp.397
Newman, D. J., & Cragg, G. M. (2012). Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of natural products, 75(3), 311-335. https://doi.org/10.1021/np200906s
Park, J. E., Kim, Y. K., Kim, S. Y., Choi, J. B., Bae, T. S., Jang, Y. S., & Lee, M. H. (2023). Biocompatibility and Antibacterial Effect of Ginger Fraction Loaded PLGA Microspheres Fabricated by Coaxial Electrospray. Materials, 16(5), 1885. https://doi.org/10.3390/ma16051885
Rajpoot, S. R., Ahmad, K., Asif, H. M., Madni, M. A., Tasleem, M. W., Zafar, F., ... & Hayee, A. (2023). Study of anti-inflammatory and anti-arthritic potential of curcumin-loaded Eudragit L100 and hydroxy propyl methyl cellulose (HPMC) microparticles. Polymer Bulletin, 1-16. https://doi.org/10.1007/s00289-023-04899-y
Shreya, S., Jain, S. K., Guru, S. K., & Sahu, A. N. (2023). Anti-cancer Potential of Pleurotus Mushroom: Detailed Insight on the Potential Bioactive Molecules, In vitro-In vivo Studies, and Formulation. Letters in Drug Design & Discovery, 20(4), 439-456. https://doi.org/10.2174/1570180819666220518100010
Tare, H. L., &Thube, B. B. (2009). Comparative cardiotonic activity of C. angustifolia with digoxin on perfused frog heart. IJPRD. 2009; 1 (1): 1, 14.
Yang, C., Zhang, Z., Gan, L., Zhang, L., Yang, L., & Wu, P. (2023). Application of Biomedical Microspheres in Wound Healing. International Journal of Molecular Sciences, 24(8), 7319. https://doi.org/10.3390/ijms24087319
Zahid, N., Ejaz, M., Khalid, N., Ikram, N., Anwar, F., & Farooq, Z. (2023). Effect of Cassia Fistula Bark Hexane Extract on Lipid Parameters in Male Diabetic Rats. Esculapio Journal of SIMS, 19(01), 82-85. https://doi.org/10.51273/esc23.2519117
Zhu, Y., Chen, T., Feng, T., Zhang, J., Meng, Z., Zhang, N., ... & Zhou, Y. (2023). Fabrication and Biological Activities of All-in-One Composite Nanoemulsion Based on Blumea balsamifera Oil-Tea Tree Oil. Molecules, 28(15), 5889. https://doi.org/10.3390/molecules28155889
Zlatić, N., & Stanković, M. (2020). Anticholinesterase, Antidiabetic and anti-inflammatory activity of secondary metabolites of Teucrium species. Teucrium Species: Biology and Applications, pp. 391-411. https://doi.org/10.1007/978-3-030-52159-2_1
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