Preclinical evaluation of the diabetic wound healing activity of phytoconstituents extracted from Ficus racemosa Linn. leaves
Abstract
Human body has several multi-layered organs, but skin is one of biggest and easiest to access. It serves as body's primary line of defense alongside various skin diseased conditions. Despite receiving sufficient and appropriate care, diabetes wounds heal slowly and may take a week to complete. A progression of connective tissue patch up is the body's natural defense against tissue damage. Fresh leaves of Ficus racemosa were utilized for the study. In this study, two distinct models were employed to compare how well different Ficus racemosa leaf extracts healed wounds. Excision wounds healed more quickly and to a greater extent after being treated with a flavonoid and tannin fraction of Ficus racemosa leaf extract, suggesting improved epithelization. The extract-treated groups also experienced an increase in breaking potency of incision wounds made; higher breaking strength indicates better wound healing. Complete closure of wound of flavonoid fraction and in fraction of Ficus racemosa extract occurred in 16 and 17 days respectively. Standard treatment increased tensile strength in the diabetic linear incision wound model, followed by treatment with the flavonoid fraction and tannin fraction of Ficus racemosa leaves extract. Ultimate finding and outcome of the present study experimentally demonstrates that extracts of the flavonoid and tannin fractions of Ficus racemosa have wound-healing properties and are effective in treating diabetic wounds. From this study, we state that Ficus racemosa flavonoid fraction and tannin fraction extract has a beneficial effect on blood glucose levels, which shows hypoglycaemic activity.
References
Albahri, G., Badran, A., Hijazi, A., Daou, A., Baydoun, E., Nasser, M., & Merah, O. (2023). The therapeutic wound healing bioactivities of various medicinal plants. Life, 13(2), 317. https://doi.org/10.3390/life13020317
Bhati, R., Singh, A., Saharan, V. A., Ram, V., & Bhandari, A. (2011). Pharmacognostical standardization, extraction and anti-diabetic activity of Smilax china L. rhizome. Asian J. Tradit. Med., 6(5), 218-223.
Biswas, T. K., & Mukherjee, B. (2003). Plant medicines of Indian origin for wound healing activity: a review. The International Journal of Lower Extremity Wounds, 2(1), 25-39.
Burgess, J. L., Wyant, W. A., Abdo Abujamra, B., Kirsner, R. S., & Jozic, I. (2021). Diabetic wound-healing science. Medicina, 57(10), 1072. https://doi.org/10.3390/medicina57101072
Chopra, D. (2014). Anaphylaxis: case report. Reactions, 1503, 38-31.
Chauhan, S., Chalotra, R., Rathi, A., Saini, M., Deol, S., Lard, M., & Gupta, S. (2023). Current Approaches in Healing of Wounds in Diabetes and Diabetic Foot Ulcers. Current Bioactive Compounds, 19(3), 104-121. https://doi.org/10.2174/1573407218666220823111344
Criollo-Mendoza, M. S., Contreras-Angulo, L. A., Leyva-López, N., Gutiérrez-Grijalva, E. P., Jiménez-Ortega, L. A., & Heredia, J. B. (2023). Wound healing properties of natural products: Mechanisms of action. Molecules, 28(2), 598. https://doi.org/10.3390/molecules28020598
Ghesmati, F., Firoozi, M., & Varaeii, S. (2018). Studying the effect of Aloe vera ointment on wound healing of CABG surgery in diabetic patients. J. Res. Med. Dent. Sci., 6, 256-260. https://doi.org/10.24896/jrmds.20186141
Gupta, A., Upadhyay, N. K., Sawhney, R. C., & Kumar, R. (2008). A poly‐herbal formulation accelerates normal and impaired diabetic wound healing. Wound Repair and Regeneration, 16(6), 784-790. https://doi.org/10.1111/j.1524-475X.2008.00431.x
Hu, N., Cai, Z., Jiang, X., Wang, C., Tang, T., Xu, T., ... & Cui, W. (2023). Hypoxia-pretreated ADSC-derived exosome-embedded hydrogels promote angiogenesis and accelerate diabetic wound healing. Acta Biomaterialia, 157, 175-186. https://doi.org/10.1016/j.actbio.2022.11.057
Khaire, M., Bigoniya, J., & Bigoniya, P. (2023). An Insight into the Potential Mechanism of Bioactive Phytocompounds in the Wound Management. Pharmacognosy Reviews, 17(33), 43-68. https://doi.org/10.5530/097627870153
Khandelwal, K.R. (2006). Practical pharmacognosy: techniques and experiments. 10th edition, Nirali Prakashan, Pune, 149-150.
Maalej, H., Maalej, A., Bayach, A., Zykwinska, A., Colliec-Jouault, S., Sinquin, C., ... & Nasri, M. (2023). A novel pectic polysaccharide-based hydrogel derived from okra (Abelmoschus esculentus L. Moench) for chronic diabetic wound healing. European Polymer Journal, 183, 111763. https://doi.org/10.1016/j.eurpolymj.2022.111763
Moses, R. L., Prescott, T. A., Mas-Claret, E., Steadman, R., Moseley, R., & Sloan, A. J. (2023). Evidence for natural products as alternative wound-healing therapies. Biomolecules, 13(3), 444. https://doi.org/10.3390/biom13030444
Pahari, N., Majumdar, S., Karati, D., & Mazumder, R. (2022). Exploring the pharmacognostic properties and pharmacological activities of phytocompounds present in Ficus Racemosa Linn.: a concise review. Pharmacological Research-Modern Chinese Medicine, 100137. https://doi.org/10.1016/j.prmcm.2022.100137
Pandian, C., Srinivasan, A., & Pelapolu, I. C. (2013). Evaluation of wound healing activity of hydroalcoholic extract of leaves of Stachytarpheta jamaicensis in streptozotocin induced diabetic rats. Der Pharmacia Lettre, 5(2), 193-200.
Pirbalouti, A. G., Azizi, S., Koohpayeh, A., & Hamedi, B. (2010). Wound healing activity of Malva sylvestris and Punica granatum in alloxan-induced diabetic rats. Acta Pol. Pharm., 67(5), 511-516.
Sharma, Y., Jeyabalan, G., Singh, R., & Semwal, A. (2013). Current aspects of wound healing agents from medicinal plants: a review. Journal of Medicinal Plants Studies, 1, 2320-3862.
Suresh Babu, A. R., & Karki, S. S. (2012). Wound healing activity of calotropis gigantea leaves in albino wistar rats. International Journal of Pharmacy, 2(1), 195-199.
Xiang, Y., Qi, X., Cai, E., Zhang, C., Wang, J., Lan, Y., ... & Hu, R. (2023). Highly efficient bacteria-infected diabetic wound healing employing a melanin-reinforced biopolymer hydrogel. Chemical Engineering Journal, 460, 141852. https://doi.org/10.1016/j.cej.2023.141852
Zuber, M., Rajesh, V., Anusha, K., Reddy, C. R., & Tirupathi, A. (2013). Wound healing activity of ethanolic extract of Allium sativum on alloxan induced diabetic rats family (Liliaceae). International Journal of Science Inventions Today, 2(1), 40-57.
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