A comprehensive study on the assessment of chemically modified Azolla pinnata as a potential cadmium sequestering agent

Kaakarlu Shivakumar Vinanthi Rajalakshmi; Kuppusamy Alagesan Paari

doi:10.52756/ijerr.2023.v36.001

Kaakarlu Shivakumar Vinanthi Rajalakshmi Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India https://orcid.org/0009-0005-7427-5239
Kuppusamy Alagesan Paari Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India https://orcid.org/0000-0002-6080-4137

DOI: https://doi.org/10.52756/ijerr.2023.v36.001

Keywords: Azolla pinnata, bioactive profile, bioremediation, cadmium chelation, chemical treatment, surface modifications

Abstract

The major environmental issue raised throughout the world is the egression of toxic pollutants in water bodies. Hence, employment of novel technological interventions such as bioremediation and phytoremediation for mitigating the toxic effects caused by the pollutants has gained attention. The aquatic macrophyte, Azolla pinnata is utilized as a biofiltering agent in the present study for the chelation of metal toxicants from the artificial wastewater system. The nutritive value of A. pinnata was determined to be 268.99Kcal/100g energy and the mineral profiling showed the highest amount of calcium (54.7ppm), iron (14.04ppm) and manganese (7.96 ppm). The quantitative screening of total phenolic and total flavonoid contents showed a maximum of 402.33±4.29 mg/g GAE and 105.25±3.81 mg/g QE respectively and the sample exhibited strong antioxidant activity in quenching the DPPH radicals with an IC₅₀ value of 88.27μg/ml. Similarly, the highest bioactivity was observed in methanolic and chloroform extract of A. pinnata biomass showing the zone of growth inhibition against E. coli (17mm) and S. aureus (18mm). The results recorded from the SEM-EDX, GCMS, FTIR and XRD confirmed the adsorptive properties of biomass. The chemically modified and unmodified Azolla exposed to cadmium metal solution showed the maximum adsorption of about 0.47±0.001 and 0.48±0.003 ppm in 60mins using the unmodified biomass with dosage of 0.75 and 1.0g respectively. Moreover, the results recorded from the instrumental characterization for the adsorptive properties of Azolla biomass proved that cadmium chelation is due to the modifications caused in porosity, surface structure and the addition of functional groups in the treated biomass surface.

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