Antidiabetic and antihyperlipidemic effects of crude fractions from Chlorophytum borivilianum root methanolic extract on streptozotocin induced diabetic rats and phytochemical investigation by LCMS analysis.

Sunayana Vikhe; Pravin Aladi; Rahul Laxman Vikhe

doi:10.52756/ijerr.2024.v38.003

Authors

Sunayana Vikhe Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni, Maharashtra, India https://orcid.org/0000-0001-5515-4005
Pravin Aladi Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni, Maharashtra, India https://orcid.org/0000-0001-5515-4005
Rahul Laxman Vikhe Department of Financial Management, Sir Vishveshwaraiya Institute of Technology, Sinnar, Maharashtra, India https://orcid.org/0000-0001-5515-4005

DOI:

https://doi.org/10.52756/ijerr.2024.v38.003

Keywords:

α-amylase, α-glucosidase, diabetes, hyperlipidemia, Chlorophytum borivilianum, root

Abstract

To evaluate the in vitro and in vivo pharmacological efficacy of the plant Chlorophytum borivilianum in diabetes and hyperlipidemia and to confine and describe the synthetic constituents from the roots that are in charge of the action. The present study was carried out to investigate the ethno-medical use of Chlorophytum borivilianum root methanolic extract as a potential anti-diabetic and antihyperlipidemic agent in STZ-induced diabetic rats. Extract was tested for in vitro and in vivo biological activities. Soxhlet extraction was carried out using methanol as a solvent, and TLC and column chromatography were used for fractionation. Liquid Chromatography and Mass Spectroscopic study confirmed the structures of isolated compounds. Chlorophytum borivilianum root methanolic extract showed the presence of phytoconstituents as Dihydrocapsaicin, Reserpine, Deserpidine, Biliverdin-IX-?, and Cassiamin C having a therapeutic effect. Dihydrocapsaicin was identified at RT 7.572 and the Chlorophytum borivilianum root chloroform methanolic extract fraction noticeably depleted increased blood glucose levels and had positive effects on altered lipid profile after administering a dose of 150 mg/kg orally compared with oral hypoglycemic drug metformin. All the results are dose-dependent. Active chloroform-methanol fraction from methanol extract showed the presence of anti-diabetic compound, Dihydrocapsaicin. The chloroform-methanol fraction from the methanolic extract of Chlorophytum borivilianum root can inhibit the parameters linked to diabetes and hyperlipidemia.

References

Acharya, C.K., Das, B., Madhu, N.R., Sau, S., Manna De, M., & Sarkar, B. (2023). A Comprehensive Pharmacological Appraisal of Indian Traditional Medicinal Plants with Anti-diabetic Potential. Springer Nature Singapore Pte Ltd., Advances in Diabetes Research and Management, pp. 163–193, Online ISBN-978-981-19-0027-3. https://doi.org/10.1007/978-981-19-0027-3_8

Aloke, C., Egwu, C. O., Aja, P. M., Obasi, N. A., Chukwu, J., Akumadu, B. O., Ogbu, P. N., & Achilonu, I. (2022). Current Advances in the Management of Diabetes Mellitus. Biomedicines, 10(10), 2436. https://doi.org/10.3390/biomedicines10102436

Basu, B.D., & Kirtikar, K.R. (1987). Indian Medicinal Plants, Lalit Mohan Basu publications, Allahabad, pp. 2508 – 2509.

Baye, Y.A., Getinet, M.A., Kefyalew, A.G., Abyot, E.G., Alemshet, Y. B., Tewachew, A., & Getaye, T. D. (2022). Evaluation of the Antidiabetic Activity of Hydromethanolic Roots Extracts of Rumex abyssinicus Jacq: (Polygonaceae) in Swiss Albino Mice. Evidence-Based Complementary and Alternative Medicine, 2022, 5193250, 11 pages. https://doi.org/10.1155/2022/5193250

Biswas, T., Behera, B. K., & Madhu, N.R. (2023). Technology in the Management of Type 1 and Type 2 Diabetes Mellitus: Recent Status and Future Prospects. 26 pages, Springer Nature Singapore Pte Ltd., Advances in Diabetes Research and Management. pp. 111–136. Online ISBN-978-981-19-0027-3. https://doi.org/10.1007/978-981-19-0027-3_6

Daou, M., Elnaker, N. A., Ochsenkühn, M. A., Amin, S. A., Yousef, A. F., & Yousef, L. F. (2022). In vitro α-glucosidase inhibitory activity of Tamarix nilotica shoot extracts and fractions. PloS One, 17(3), e0264969. https://doi.org/10.1371/journal.pone.0264969

Deore, S., & Khadabadi, S. (2008). Standardisation and pharmaceutical evaluation of Chlorophytum borivilianum mucilage. Rasayan Journal of Chemistry, 1.

Dhakar, S., & Tare, H. (2023). Therapeutic Potential of Polyherbal Tablets: A Comprehensive Assessment of Pharmacological Activity. Int. J. Exp. Res. Rev., 34(Special Vol.), 97-105. https://doi.org/10.52756/ijerr.2023.v34spl.010

Dixit, V.K., & Mayank, T. (2008). A review on some important medicinal plants of Chlorophytum spp., Plant Rev., 2, 168-172.

Eskandani, M., Bahadori, M.B., Zengin, G., Dinparast, L., & Bahadori, S. (2016). Novel Natural Agents from Lamiaceae Family: An Evaluation on Toxicity and Enzyme Inhibitory Potential linked to Diabetes Mellitus. Current Bioactive Compounds, 12, 34-38. https://doi.org/10.2174/1573407212666151231183118

Gokhle, S.B., Kokate, C. K., & Purohit, A. P. (2007). Pharmacognosy, nirali prakashan, pune, 37th ed., pp.105-108.

Jaiswal, S., & Gupta, P. (2023). GLSTM: A novel approach for prediction of real & synthetic PID diabetes data using GANs and LSTM classification model. Int. J. Exp. Res. Rev., 30, 32-45. https://doi.org/10.52756/ijerr.2023.v30.004

Iyangar, M.A. (1986). Pharmacognosy of powdered cruds drugs, reprint IInd ED., Published by Iyangar M.A., Kasturba Medical College, Manipal.

Kaushik, N. (2005). Saponins of Chlorophytum species. Phytochem, Rev., 4, 191-196.

Khandelwal, K.R. (2005). Practical Pharamacognosy 14th Edn., Nirali Prakashan, Pune. 149, 157.

Kokate, C.K. (1989). Practical pharmacognosy, IInd Ed., Nirali Prakashan, Pune.

Kunkulol, R., & Vikhe, S. (2020). Microscopic Investigations and Pharmacognosy of Striga orobanchioides Benth. Pharmacogn. J., 12(6), 1325-1331.

Pharmacopoeia of India (1966). IInd Ed., published by Controller of Publication, Govt. of India, New Delhi pp. 947.

Pramanik, B. (2018). A comparative study on the knowledge, attitude and risk perception regarding complications of type-2 diabetes mellitus between male and female diabetic patients attending diabetic clinics in selected hospital of West Bengal, India. Int. J. Exp. Res. Rev., 15, 16-27. https://doi.org/10.52756/ijerr.2018.v15.004

Remok, F., Saidi, S., Gourich, A.A., Zibouh, K., Maouloua, M., Makhoukhi, F.E., Menyiy, N.E., Touijer, H., Bouhrim, M., & Sahpaz, S. (2023). Phenolic Content, Antioxidant, Antibacterial, Antihyperglycemic, and α-Amylase Inhibitory Activities of Aqueous Extract of Salvia lavandulifolia Vahl. Pharmaceuticals, 16(3), 395. https://doi.org/10.3390/ph16030395

Roy, R., Chakraborty, A., Jana, K., Sarkar, B., Biswas, P., & Madhu, N.R. (2023). The Broader Aspects of Treating Diabetes with the Application of Nanobiotechnology. Springer Nature Singapore Pte Ltd., Advances in Diabetes Research and Management, pp. 137–162, Online ISBN-978-981-19-0027-3, https://doi.org/10.1007/978-981-19-0027-3_7

Safitri, A., Tirto Sari, D. R., Refsilangi, B., Roosdiana, A., & Fatchiyah, F. (2021). Histopathological Profiles of Rats (Rattus norvegicus) Induced with Streptozotocin and Treated with Aqueous Root Extracts of Ruellia tuberosa L. Veterinary Medicine International, 2021, 6938433.

https://doi.org/10.1155/2021/6938433

Sarkar, B., Bhattacharya, P., Yen Chen, C., Maity, J., & Biswas, T. (2022). A comprehensive characterization and therapeutic properties in ripened Noni fruits (Morinda citrifolia L.). International Journal of Experimental Research and Review, 29, 10-32. https://doi.org/10.52756/ijerr.2022.v29.002

Sarkar, S., Sadhu, S., Roy, R., Tarafdar, S., Mukherjee, N., Sil, M., Goswami, A., & Madhu, N.R. (2023). Contemporary Drifts in Diabetes Management. Int. J. App. Pharm., 15(2), 1-9. https://doi.org/10.22159/ijap.2023v15i2.46792

Srivastava, A.K., Mukerjee, A. & Tripathi, A. (2020). Antidiabetic and antihyperlipidemic activities of Cucumis melo var. momordica fruit extract on experimental animals. Futur. J. Pharm. Sci., 6, 92. https://doi.org/10.1186/s43094-020-00116-z

Sunayana, V., & Sunil, N. (2018). Antiallergic and antihistaminic actions of Ceasalpinia bonducella seeds: Possible role in treatment of asthma. Journal of Ethnopharmacology, 216, 251-258. https://doi.org/10.1016/j.jep.2017.12.007.

Sundaram, S., Dwivedi, P., & Purwar, S. (2011). Antibacterial Activities of Crude Extracts of Chlorophytum borivilianum to Bacterial Pathogens. Research Journal of Medicinal Plant, 5, 343-347. https://doi.org/10.3923/rjmp.2011.343.347.

Sur, T., Das, A., Bashar, S., Tarafdar, S., Sarkar, B., & Madhu, N.R. (2023). Biochemical Assay for Measuring Diabetes Mellitus. Springer Nature Singapore Pte Ltd., Advances in Diabetes Research and Management, pp. 1–20, Online ISBN-978-981-19-0027-3, https://doi.org/10.1007/978-981-19-0027-3_1

Tandon, M., Thakur, R. S., & Shukla, Y. N. (1992). 4-Hydroxy, 8-11 oxidoheneicosanol and other constituents from Chlorophytum arundinaceum roots. Phytochemistry, 31, 2525-2526.

Telagari, M., & Hullatti, K. (2015). In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions. Indian Journal of Pharmacology, 47(4), 425–429. https://doi.org/10.4103/0253-7613.161270

The Indian Pharmacopoea (1966). 2nd Edn., Govt. of India, publications, Delhi. pp.947 – 948.

Tyagi, K., Kumar, D., & Gupta, R. (2024). Application of Genetic Algorithms for Medical Diagnosis of Diabetes Mellitus. International Journal of Experimental Research and Review, 37(Special Vo), 1-10. https://doi.org/10.52756/ijerr.2024.v37spl.001