The Use of Ultrasonics for Stable Emulsion Preparation of Fir and Cedar Essential Oils

Maryia D Hurda; Tamara P Arseneva; Daria A Fomicheva; Mariia A Antcyperova; Artem I Lepeshkin; Natalia V Iakovchenko

doi:10.52756/ijerr.2024.v46.006

Authors

Maryia D Hurda ITMO University, Faculty of Biotechnologies, Kronverksky prospect 49, 197101 Saint Petersburg, Russian Federation https://orcid.org/0000-0001-6463-8036
Tamara P Arseneva ITMO University, Faculty of Biotechnologies, Kronverksky prospect 49, 197101 Saint Petersburg, Russian Federation https://orcid.org/0009-0005-1716-4583
Daria A Fomicheva ITMO University, Faculty of Biotechnologies, Kronverksky prospect 49, 197101 Saint Petersburg, Russian Federation https://orcid.org/0000-0002-1770-4456
Mariia A Antcyperova ITMO University, Faculty of Biotechnologies, Kronverksky prospect 49, 197101 Saint Petersburg, Russian Federation https://orcid.org/0009-0004-0005-6597
Artem I Lepeshkin ITMO University, Faculty of Biotechnologies, Kronverksky prospect 49, 197101 Saint Petersburg, Russian Federation https://orcid.org/0000-0001-9118-1449
Natalia V Iakovchenko ITMO University, Faculty of Biotechnologies, Kronverksky prospect 49, 197101 Saint Petersburg, Russian Federation https://orcid.org/0000-0002-5188-5916

DOI:

https://doi.org/10.52756/ijerr.2024.v46.006

Keywords:

Cedar essential oil, Emulsifying agents, Emulsion, Emulsion stability, Fir essential oil

Abstract

The properties of fir and cedar essential oils are very diverse. The main ones are antibacterial, anti-inflammatory, antifungal and antiviral. Furthermore, fir and cedar essential oils help to speed up metabolism and reduce stress. Currently, a lot of research is being done on the anti-cancer properties of these oils. The developed nanoemulsion makes it possible to use all the valuable substances of fir and cedar essential oils. Products with it can be used to prevent neoplasms in the human body and improve the functioning of the muscular system and individual organs, such as the liver. The interest in ultrasonic processing is based on the beneficial properties it brings to food products, particularly, disinfection, emulsification, intensification of some technological processes, and so on. Various creams, suspensions and emulsions are produced using ultrasonic processing. All over the world, research is underway to obtain and study the properties of these solutions. In this study, a technology for obtaining a stable nanoemulsion (oil-water) using ultrasonic vibrations has been developed. The results showed that the nanoemulsion with using Tween 80 emulsifier is the most stable. The rational ratio of oil: emulsifier: water was determined. The optimal surfactant for obtaining a stable emulsion is the TWEEN 80 emulsifier at a concentration twice the concentration of oils - the ratio of emulsifier to oil is 1:2, respectively. The appearance and formula of the emulsion obtained by ultrasonic generator is much better than that obtained by dispersion and mixing. To obtain a stable emulsion, treatment with an ultrasonic generator at a frequency of 22 kHz, power of 100% and processing in an ice bath is necessary. A comparative analysis of the appearance and stability of the obtained nanoemulsion was carried out in comparison with nanoemulsions obtained by other methods. This nanoemulsion can be used in the food industry to improve the antibacterial activity in food products, prolong the shelf life, and increase the nutritional value. Moreover, developed nanoemulsion can be widely used in the cosmetic and pharmaceutical industries for both oral preparations and transdermal use.

References

Baitukalov, T. A., Bogoslovskaya, O.A., Glushenko, N.N., Orekhova, O.I., & Olkhovskaya, I.P. (2004). Physical and chemical indicators of cedar and linseed oils and the formation of dosage forms based on them. Vestnik RUDN University, 4, 253–256. (in Russian)

Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94(3), 223–253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022

Carson, C. F., Hammer, K. A., & Riley, T. V. (2006). Melaleuca alternifolia (Tea Tree) Oil: a Review of Antimicrobial and Other Medicinal Properties. Clinical Microbiology Reviews, 19(1), 50–62. https://doi.org/10.1128/cmr.19.1.50-62.2006

Cheng, S. (2003). Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae. Bioresource Technology, 89(1), 99–102. https://doi.org/10.1016/s0960-8524(03)00008-7

De Temmerman, M.L., Dewitte, H., Vandenbroucke, R. E., Lucas, B., Libert, C., Demeester, J., Smedt, S. C. D., Lentacker, I., & Rejman, J. (2011). mRNA-Lipoplex loaded microbubble contrast agents for ultrasound-assisted transfection of dendritic cells. Biomaterials, 32(34), 9128–9135. https://doi.org/10.1016/j.biomaterials.2011.08.024

Hawkins, A. N., Licea, S. J., Sleeper, S. A., & Swearingen, M. C. (2022). Calcium sulfate beads made with antibacterial essential oil-water emulsions exhibit growth inhibition against Staphylococcus aureus in agar pour plates. PLoS One, 17(7), e0271209. https://doi.org/10.1371/journal.pone.0271209

Huang, K., Liu, R., Zhang, Y., & Guan, X. (2021). Characteristics of two cedarwood essential oil emulsions and their antioxidant and antibacterial activities. Food Chemistry, 346, 128970. https://doi.org/10.1016/j.foodchem.2020.128970

Ka?ániová, M., Galovi?ová, L., Valková, V., ?uranová, H., Štefániková, J., ?miková, N., Vukic, M., Vukovic, N. L., & Kowalczewski, P. ?. (2022). Chemical Composition, Antioxidant, In Vitro and In Situ Antimicrobial, Antibiofilm, and Anti-Insect Activity of Cedar atlantica Essential Oil. Plants, 11(3), 358. https://doi.org/10.3390/plants11030358

Koul, O., Walia, S., & Dhaliwal, G.S. (2008). Essential oils as green pesticides: potential and constraints. Biopesticides International, 4(1), 63–84.

Lemnaru, G-M., Motelica, L., Trusca, R. D., Ilie, C. I., Croitoru, A-M., Ficai, D., Oprea, O., Stoica-Guzun, A., Ficai, A., Ditu, L-M., & Tih?uan, B.-M. (2023). Antimicrobial Wound Dressings based on Bacterial Cellulose and Independently Loaded with Nutmeg and Fir Needle Essential Oils. Polymers, 15(17), 3629. https://doi.org/10.3390/polym15173629

Maurya, S., Marimuthu, P., Singh, A., Rao, G.P., & Singh, G. (2005). Antiviral activity of essential oils and acetone extracts of medicinal plants against papaya ring spot virus. J. Essent. Oil Bear. Plants, 8, 233–238. https://doi.org/10.1080/0972060x.2005.10643452

Mediratta, P. K., Sharma, K. K. & Singh, S. (2002). Evaluation of immunomodulatory potential of Ocimum sanctum seeds oil and its possible mechanism of action. J. Ethnopharmacol, 80, 15–20. https://doi.org/10.1016/S0378-8741(01)00373-7

Mei, L., Shi, L., Song, X., Liu, S., Cheng, Q., Zhu, K., & Zhuge, R. (2021). Characterization of Carboxymethyl Cellulose Films Incorporated with Chinese Fir Essential Oil and Their Application to Quality Improvement of Shine Muscat Grape. Coatings, 11, 97. https://doi.org/10.3390/coatings11010097

Nedorostova, L., Kloucek, P., Kokoska, L., Stolcova, M, & Pulkrabek, J. (2009). Antimicrobial properties of selected essential oils in vapour phase against food borne bacteria. Food Control, 20, 157–160. https://doi.org/10.1016/j.foodcont.2008.03.007

Nuutila, A. M., Puupponen-Pimiä, R., Aarni, M., & Oksman-Caldentey, K. M. (2003). Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scave. Food Chemistry, 81(4), 485–493. https://doi.org/10.1016/S0308-8146(02)00476-4

Otero, V., Becerril, R., Santos, J. A., Rodríguez-Calleja, J. M., Nerín, C., & García-López, M. L. (2014). Evaluation of two antimicrobial packaging films against Escherichia coli O157: H7 strains in vitro and during storage of a Spanish ripened sheep cheese (Zamorano). Food Control, 42, 296–302. https://doi.org/10.1016/j.foodcont.2014.02.022

Pandey, A. K., Kumar, P., Singh, P., Tripathi, N. N., & Bajpai, V. K. (2017). Essential Oils: Sources of Antimicrobials and Food Preservatives. Frontiers in Microbiology, 7, 1-14. https://doi.org/10.3389/fmicb.2016.02161

Pandey, A. K., Palni, U. T., & Tripathi, N. N. (2014). Repellent activity of some essential oils against two stored product beetles Callosobruchus chinensis L. and C. maculates F. (Coleoptera: Bruchidae) with reference to Chenopodium ambrosioides L. for the safety of pigeon pea seeds). J. Food Sci. Technol, 51, 4066–4071. https://doi.org/10.1007/s13197-012-0896-4

Pandey, A. K., Sonker, N., & Singh, P. (2016). Efficacy of Some Essential Oils Against Aspergillus flavus with Special Reference toLippia albaOil an Inhibitor of Fungal Proliferation and Aflatoxin B1Production in Green Gram Seeds during Storage. Journal of Food Science, 81(4), 928–934. https://doi.org/10.1111/1750-3841.13254

Paniwnyk, L. (2017). Applications of ultrasound in processing of liquid foods: A review. Ultrasonics Sonochemistry, 38, 794–806. https://doi.org/10.1016/j.ultsonch.2016.12.025

Perdones, Á., Escriche, I., Chiralt, A., & Vargas, M. (2016). Effect of chitosan-lemon essential oil coatings on volatile profile of strawberries during storage. Food Chemistry, 197, 979-986. https://doi.org/10.1016/j.foodchem.2015.11.054

Poaty, B., Lahlah, J., Porqueres, F., & Bouafif, H. (2015). Composition, antimicrobial and antioxidant activities of seven essential oils from the North American boreal forest. World Journal of Microbiology and Biotechnology, 31(6), 907–919. https://doi.org/10.1007/s11274-015-1845-y

Ramadass, M., Hakeem, S.A., Chandran, A.Y., Vadivelu, G., & Thiagarajan, P. (2019). Formulation and Characterization of Cedrus deodara Oil Emulsion and studies on its activity against representative food and plant pathogens. Research Journal of Pharmacy and Technology, 12(3), 1333. https://doi.org/10.5958/0974-360X.2019.00223.3

Reyes-Jurado, F., Navarro-Cruz, A. R., Ochoa-Velasco, C. E., Palou, E., López-Malo, A., & Ávila-Sosa, R. (2019). Essential oils in vapor phase as alternative antimicrobials: A review. Critical Reviews in Food Science and Nutrition, 60(3), 1-10. https://doi.org/10.1080/10408398.2019.1586641

Prosekov, A., Dyshlyuk, L., Milent'eva, I., Pavsky, V., Ivanova, S., & Garmashov, S. (2018). Study of the biofunctional properties of cedar pine oil with the use of in vitro testing cultures. Foods and Raw Materials, 6(1), 136-143. https://doi.org/10.21603/2308-4057-2018-1-136-143

Reische, D., Lillard, D., & Eitenmiller, R. (2008). Antioxidants. Food Lipids, 3, 930-930. https://doi.org/10.1201/9780203908815.ch15

Roldan-Cruz, C., Vernon-Carter, E. J., & Alvarez-Ramirez, J. (2016). Assessing the stability of Tween 80-based O/W emulsions with cyclic voltammetry and electrical impedance spectroscopy. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 511, 145–152. https://doi.org/10.1016/j.colsurfa.2016.09.074

Sanders, C., Diego, M., Fernandez, M., Field, T., Hernandez-reif, M., & Roca, A. (2002). Eeg asymmetry responses to lavender and rosemary aromas in adults and infants. International Journal of Neuroscience, 112(11), 1305–1320. https://doi.org/10.1080/00207450290158214

Sivakumar, M., Tang, S. Y., & Tan, K. W. (2014). Cavitation technology – A greener processing technique for the generation of pharmaceutical nanoemulsions. Ultrasonics Sonochemistry, 21(6), 2069–2083. https://doi.org/10.1016/j.ultsonch.2014.03.025

Song, X., Shi, L., Liu, S., Hou, C., Zhu, K., Cheng, Q., & Mei, L. (2022). Development of Microcapsule Bioactive Paper Loaded with Chinese Fir Essential Oil to Improve the Quality of Strawberries. Coatings, 12(2), 254. https://doi.org/10.3390/coatings12020254

Taghavi, E., Abdul Rahman, F. N., Lani, M. N., Hamzah, Y., Anarjan, N., & MohdMaidin, N. (2023). Effect of Environmental Stress on Physical Properties and Antibacterial Activity of Atlas Cedar (Cedrus atlantica) Oil-in-water Emulsion. Journal of Medicinal Plants & By-Products, 13(1), 31. https://doi.org/10.22034/jmpb.2023.128390

Tamer, T. M., Sabet, M. M., Alhalili, Z. A. H., Ismail, A. M., Mohy-Eldin, M. S., & Hassan, M. A. (2022). Influence of Cedar Essential Oil on Physical and Biological Properties of Hemostatic, Antibacterial, and Antioxidant Polyvinyl Alcohol/Cedar Oil/Kaolin Composite Hydrogels. Pharmaceutics, 14(12), 2649. https://doi.org/10.3390/pharmaceutics14122649

Ye, C.-L., Dai, D.-H., & Hu, W.-L. (2013). Antimicrobial and antioxidant activities of the essential oil from onion (Allium cepa L.). Food Control, 30(1), 48-53. https://doi.org/10.1016/j.foodcont.2012.07.033

Wang, Y., Ai, C., Wang, H., Chen, C., Teng, H., Xiao, J., & Chen, L. (2023). Emulsion and its application in the food field: An update review. Efood, 4(4), e102. https://doi.org/10.1002/efd2.102

Wi?ska, K., M?czka, W., ?yczko, J., Grabarczyk, M., Czubaszek, A., & Szumny, A. (2019). Essential Oils as Antimicrobial Agents—Myth or Real Alternative? Molecules, 24(11), 2130. https://doi.org/10.3390/molecules24112130

Zhang, Y., Han, L., Chen, S.-S., Guan, J., Qu, F.-Z., & Zhao, Y.Q. (2016). Hair growth promoting activity of cedrol isolated from the leaves of Platycladus orientalis. Biomedicine & Pharmacotherapy, 83, 641–647. https://doi.org/10.1016/j.biopha.2016.07.022

Zhang, Y., Han, L., Chen, S.S., Huang, X.F., Chang, K.F., Lee, S.C., Sheu, G.T., Li, C.Y., Weng, J.-C., Hsiao, C.-Y., & Tsai, N.M. (2020). Extract Derived from Cedrus atlantica Acts as an Antitumor Agent on Hepatocellular Carcinoma Growth In Vitro and In Vivo. Molecules, 25(20), 4608. https://doi.org/10.3390/molecules25204608

Zrira, S., & Ghanmi, M. (2016). Chemical Composition and Antibacterial Activity of the Essential of Cedrus atlantica (Cedarwood oil). Journal of Essential Oil Bearing Plants, 19(5), 1267–1272. https://doi.org/10.1080/0972060x.2015.1137499