Analysing the association of Helicobacter pylori induced gastritis with neutrophil-lymphocyte ratio (NLR) and volume, conductance, scatter characteristics of LH780 Coulter
Abstract
Helicobacter (H.) pylori is a bacterium that infects the stomach leading to chronic gastritis. In cases of extreme infection, it can lead to condition of painful sores called peptic ulcers. To determine the association of H. pylori infection in gastric mucosa with blood parameters like Neutrophil-Lymphocyte ratio (NLR) and volume, conductance and scatter (VCS) characteristics were studied using LH780 Coulter haemogram. Blood samples of 100 normal subjects and 100-gastritis cases were collected for testing complete blood count. Biopsies of the gastric tract were also conducted for the 100 gastritis patients. The total leucocyte count was significantly higher in the gastritis patients than in the control subjects. On the other hand, the control subjects' haemoglobin percentage, RBC count, Packed Cell Volume percentage, Basophil percentage, and Lymphocyte percentage were significantly higher. Absolute neutrophil count, Neutrophil conductance (Standard Deviation, SD), Neutrophil Scatter (SD), Lymphocyte volume (SD), Lymphocyte Conductance, Lymphocyte Scatter (SD) and Neutrophil-Lymphocyte ratio were significantly high among gastritis patients. Mean neutrophil scatter was significantly higher among control subjects. Haemoglobin percentage, RBC count, PCV percentage, MCV, eosinophil percentage, basophil percentage and lymphocyte percentage were significantly higher in control subjects compared to no activity gastritis patients. Absolute neutrophil volume, neutrophil conductance (SD), lymphocyte volume (SD), lymphocyte conductance (SD), lymphocyte scatter (SD) and neutrophil-lymphocyte ratio were significantly higher among no-activity gastritis patients. RBC count, haemoglobin percentage and packed cell volume percentage were significantly higher among the control subjects compared to the moderate activity gastritis patients. Lymphocyte volume (SD) and scatter (SD) were significantly higher among moderate-activity gastritis patients. Scatter parameters such as neutrophil conductance (SD), lymphocyte conductance SD and lymphocyte scatter (SD) were significantly higher among high-activity gastritis patients. Mean neutrophil scatter was significantly higher among control subjects compared to high activity gastritis patients. Based on our observation we propose that the N/L ratio shows signs of escalation due to the increased severity of the inflammation and intensified neutrophil infiltration of the gastric mucosa.
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