Changes of gut microbiota in FAP and UC patients in Mediterranean region of Turkey: an omic landscape to be discovered

Atil Bisgin; Abdullah Hanta; Ahmet Rencuzogullari; Ismail Cem Eray; Surajit Pathak; Ibrahim Boga

doi:10.52756/ijerr.2023.v30.023

Atil Bisgin Cukurova University, AGENTEM (Adana Genetic Diseases Diagnosis and Treatment Center), Adana, Turkey; Cukurova University, Faculty of Medicine, Department of Medical Genetics, Adana, Turkey https://orcid.org/0000-0002-2053-9076
Abdullah Hanta Cukurova University, AGENTEM (Adana Genetic Diseases Diagnosis and Treatment Center), Adana, Turkey
Ahmet Rencuzogullari Koc University, School of Medicine, Department of General Surgery, Istanbul, Turkey
Ismail Cem Eray Cukurova University, Faculty of Medicine, Surgical Oncology Department, Adana, Turkey
Surajit Pathak Department of Medical Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Chennai, India
Ibrahim Boga Cukurova University, AGENTEM (Adana Genetic Diseases Diagnosis and Treatment Center), Adana, Turkey; Cukurova University, Faculty of Medicine, Department of Medical Genetics, Adana, Turkey https://orcid.org/0000-0002-8967-8218

DOI: https://doi.org/10.52756/ijerr.2023.v30.023

Keywords: Dysbiosis, Familial adenomatous polyposis, Geographic Populations, Gut Microbiota, Ulcerative Colitis, 16S rRNA

Abstract

Inflammatory bowel diseases, familial adenomatous polyposis (FAP) and colorectal cancer (CRC) are associated with alterations of the intestinal microbiota. However, few data are available on the perpetuation of FAP and ulcerative colitis (UC) in relation to microbial dysbiosis. This study evaluated the UC and genetically confirmed FAP patients’ gut microbial balance in concordance to clinical outcome. Fecal materials (average mass of 0.54 g) were collected from three FAP and five UC patients to compare with healthy individuals as control group. Genomic materials of microbiota were isolated for next generation sequencing of 16S rRNA that was performed by using QIAseq 16S/ITS panel in Illumina Miseq Platform. Data processing and bioinformatics analysis were performed via CLC Genomic Workbench bioinformatics tool. The comparison between FAP, UC and control group revealed an alteration in the intestinal microbial composition. More in details, relative abundance of class levels showed statistical significance differences among FAP, UC and control groups. Our preliminary data focused on the ex-planation of how dysbiosis can lead to inflammation and drive processes together with host genetic profile that leads to colorectal carcinogenesis.

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