Protection of hydrogen peroxide and Metal induced DNA damage by flavonoids
Abstract
More recent research has shown that free radicals cause biomolecules to oxidatively damage. This harm has been linked to several human illnesses, diseases, and ageing. Reactive oxygen species (ROS), such as the hydroxide free radical (OH), hydrogen peroxide (H2O2), and superoxide ion (O2-), have been proven to cause oxidative damage to DNA. Naringin, quercetin, and naringinin have been demonstrated to assist in DNA from oxidative damage. The 2,2-diphenylpicrylhydrazyl (DPPH) test was used to gauge the capacity to neutralize free radicals. The method used was founded on spectrophotometric evaluation of the modification in DPPH concentration caused by the interaction with an antioxidant. Based on the apparent contradiction in words, the study shows the impact of quercetin, naringin, and naringinin on DNA damage in the presence of cupric ions and H2O2 using the gel electrophoresis technique. The aforementioned flavonoids acted as a protective agent at low cupric ion concentrations. While hydroxy free radical scavenger (FRS) did not prevent DNA cleavage at greater cupric ion concentrations, flavonoids did. It follows that DNA damage brought on by flavonoids is not simply caused by oxidative stress. The current work will look at a possible mechanism for the development of Copper(II) complexes with quercetin, naringin, and naringinin that promote antioxidant activity. To properly assess the efficacy of naringenin, quercetin, and naringin when combined with chemotherapeutic drugs for the administration of human health benefits, additional in-depth investigations, including clinical trials, are required.
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