Effect of Borreria hispida Extract on SIRT1, HIF-1?, ET-1 and VEGFR-2 Gene Expression in NRK-52E Cells Subjected to Glucotoxicity

Devaprasad Markandeyan; Shiek S.S.J. Ahmed; Palani Perumal; Arulvasu Chinnasamy; Bhavatarini Govindaraj; Niranjni Sekar; Sudhan Mookandi; Priyadarshini Shanmugam; Janakiraman Velayudam; Deepakrajasekar Padmanaban; Jayaraman Perumal

doi:10.52756/ijerr.2024.v37spl.010

Authors

Devaprasad Markandeyan Department of Neurology, Faculty of Medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0002-9548-5324
Shiek S.S.J. Ahmed Drug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0003-3403-4084
Palani Perumal Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0000-0002-6774-6478
Arulvasu Chinnasamy Dept. of Zoology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0000-0002-4613-259X
Bhavatarini Govindaraj Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0000-0001-7555-9931
Niranjni Sekar Dept. of Zoology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0009-0003-8577-7408
Sudhan Mookandi Drug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0001-9117-154X
Priyadarshini Shanmugam Department of Microbiology, Faculty of Medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0003-4382-562X
Janakiraman Velayudam Drug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0003-2774-9022
Deepakrajasekar Padmanaban Dept. of Zoology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0009-0003-8632-3037
Jayaraman Perumal Department of Microbiology, Faculty of Medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0002-4170-2055

DOI:

https://doi.org/10.52756/ijerr.2024.v37spl.010

Keywords:

Borreria hispida, Diabetic kidney disease, ET-1, HIF-1λ, NRK 52E, SIRT1, VEGFR-2

Abstract

This study aimed to investigate the impact of Borreria hispida extract on gene expression in NRK-52E cells under conditions of glucotoxicity. Gene expression analysis was conducted using RT-PCR following the exposure of cultured NRK-52E cells to glucotoxic conditions and varying concentrations of Borreria hispida extract. The results demonstrated a dose-dependent increase in SIRT1 gene expression and a concomitant decrease in HIF-1?, ET-1, and VEGFR-2 gene expressions upon treatment with Borreria hispida extract. Additionally, molecular docking studies suggested the potential inhibition of Rho-kinase as a mechanistic explanation for these effects. Borreria hispida extract may confer renoprotective benefits against glucotoxicity-induced cellular damage. The potential therapeutic utility of Borreria hispida extracts in managing renal complications associated with conditions such as diabetes. Furthermore, our docking studies shed light on the potential molecular mechanisms underlying the observed effects, suggesting interactions between phytochemicals present in Borreria hispida extract and the JNK-1 protein. These interactions may contribute to the augmentation of SIRT1 activity, further bolstering the extract's therapeutic utility in DKD.

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