Effect of Orthosiphon stamineus Extract on HIF-1?, Endothelin-1, and VEGFR-2 Gene Expression in NRK-52E Renal Tubular Cells Subjected to Glucotixicity

Devaprasad Markandeyan; Shiek S.S.J. Ahmed; Palani Perumal; Arulvasu Chinnasamy; Bhavatarini Govindaraj; Niranjni Sekar; Sudhan Mookandi; Priyadarshini Shanmugam; Janakiraman Velayudam; Deepak Rajasekar Padmanaban; Perumal Jayaraman

doi:10.52756/ijerr.2024.v37spl.015

Authors

Devaprasad Markandeyan Department of Neurology, Faculty of Medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0002-9548-5324
Shiek S.S.J. Ahmed Drug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0003-3403-4084
Palani Perumal Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0000-0002-6774-6478
Arulvasu Chinnasamy Dept. of Zoology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0000-0002-4613-259X
Bhavatarini Govindaraj Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0000-0001-7555-9931
Niranjni Sekar Dept. of Zoology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0009-0003-8577-7408
Sudhan Mookandi Drug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0001-9117-154X
Priyadarshini Shanmugam Department of Microbiology, Faculty of Medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0003-4382-562X
Janakiraman Velayudam Drug Discovery and Multi-omics Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0003-2774-9022
Deepak Rajasekar Padmanaban Dept. of Zoology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India https://orcid.org/0009-0003-8632-3037
Perumal Jayaraman Department of Microbiology, Faculty of Medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India https://orcid.org/0000-0002-4170-2055

DOI:

https://doi.org/10.52756/ijerr.2024.v37spl.015

Keywords:

Orthosiphon stamineus, HIF-1α, ET-1, VEGFR-2, RHO-kinase, norstaminol_b, orthosiphol_j, orthosiphol m

Abstract

This study aimed to investigate the impact of Orthosiphon stamineus extract on gene expression in NRK-52E cells under conditions of glucotoxicity. Gene expression analysis using RT-PCR was conducted following exposure of cultured NRK-52E cells to glucotoxic conditions and various concentrations of Orthosiphon stamineus extract. The results revealed a dose-dependent decrease in HIF-1?, ET-1 and VEGFR-2 gene expression levels upon treatment with Orthosiphon stamineus extract. The diverse array of phytochemicals found within Orthosiphon demonstrates a significant impact on the biochemical pathways implicated in the pathogenesis of the disease. Additionally, molecular docking studies suggested the potential inhibition of Rho-kinase as a mechanistic explanation for this observed effect. These findings suggest that Orthosiphon stamineus extract may possess renoprotective properties against glucotoxicity-induced cellular damage. This study contributes to understanding potential therapeutic interventions for managing renal complications associated with conditions such as diabetes.

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