A Comprehensive Chemical Characterization of Leaves of Five Potential Medicinal Plants in Paschim Medinipur District, W. B., India
Abstract
The physico-chemical and spectroscopic characterization of five selected medicinal plants viz., Acalypha indica, Senna tora, Euphorbia hirta, Physalis angulata and Ziziphus mauritina are the essence and has been carried out to assess bioactive potential and to establish traditional belief in the light of scientific interpretation. Fresh leaves were collected from Paschim Medinipur district of West Bengal, India, dried and powdered for Proximate analysis, ultimate analysis and compositional analysis, FTIR and GCMS. The study revealed that the highest concentration of Volatile matter (75.452%), Bulk density (0.256 %), Swelling index (1.456 %), Cellulose content (61.727 %), Hemicellulose content (4.372 %) and Tannins (0.635 g/ 100g) was observed to occur in Acalypha indica leaf than other experimental plants. In Physalis angulata the percentage composition of fixed carbon (15.251%) and extractive content (1.974 %) was higher than others. Ash content (12.327 %), hemicellulose content (4.372 %), and lignin content (5.531 %) were observed to be higher in the Senna tora leaf than others. Total carbohydrate concentrations (9.619 gm/100 gm) and nitrogen content (2.050 %) were observed to be higher in Euphorbia hirta than others. Total protein (2.321 gm/100 gm), total fat (0.655 gm/100 gm), oxygen content (34.222 %) and Higher heat value (HHV) (16.546 %) were higher in Ziziphus mauritina than others. The existence of functional groups and the relevance of the presence of a specific bioactive component were determined using FTIR (Fourier Transform Infrared Spectroscopy) of the leaves of five plant samples. According to GCMS (Gas Chromatography Mass Spectroscopy), five different medicinal plants contain significant bioactive chemicals. By using GC-MS analysis, a total of 10 bioactive compounds (Acalypha indica, Senna tora) and 8 bioactive compounds (Ziziphus mauritina, Euphorbia hirta, Physalis Angulata) were found in the peaks of methanolic extracts of five potential medicinal plants. The bioactive compounds like Cyclotrisiloxane, Hexamethyl-, Dodecane, 1-Fluoro-, Myo-Inositol, 4-C-Methyl obtained from Acalypha indica leaf extract, Palmitic Acid, Phthalic Acid, Cyclopentadecanol obtained from Senna tora leaf extract and 4-Tert-Octylphenol, TMS Derivative obtained from Euphorbia hirta leaf extract has no records of individual bioactivity as per Dr. Duke’s ethnobotanical and pharmaceutical database and are considered as novel bioactive compounds. Isolation of these compounds and successive bioactivity studies should be the thrust area for future researchers. Also, the current study confirms that specific plant leaves are a great source of important phytochemicals and can be used to make herbal formulations, practical medications, and complementary medicines.
References
Acharya, C. K. (2015). Ethnic uses and Conservation Practices of Some Plants with Major Medicinal Potential by the Tribal Community in India. International Journal of Life Sciences, 9(6), 1 – 7. https://doi.org/10.3126/ijls.v9i6.13435
Acharya, C. K. (2016). Ethnicity and Scientific validation of West Bengal Amla (Phyllanthus emblica L.) with special reference to GC-MS screening. Int. J. Exp. Res. Rev., 3, 51- 59. https://doi.org/10.13140/RG.2.1.3922.3440
Acharya, C.K., Khan, N.S., Madhu N.R., & Guha, P. (2021b). Improved Reproductive Efficacy of Phyllanthus emblica L. (Gaertn.) on Testis of Male Swiss Mice and a Pilot Study of its Potential Values. Int. J. Food Nutr. Sci., 10(4),7-14. https://doi.org/10.4103/ijfans_34_21
Acharya, C.K., Khan, N.S., & Madhu, N.R. (2022). A comparative GC-MS analysis of bioactive compounds in ethyl acetate fruit extract of Phyllanthus emblica L. (Gaertn.) growing in two phyto-geographically contrasting regions of West Bengal, India. Jour. Pl. Sci. Res., 38(1), 343–355. https://doi.org/10.32381/JPSR.2022.38.01.37
Acharya, C.K., Khan, N.S., & Madhu, N.R. (2021a). Medicinal uses of Amla, Phyllanthus emblica L. (Gaertn.): A Prospective Review. Mukt Shabd Journal, 10(10), 296- 310.
Alam, S., Sarker, M.M.R., Sultana, T.N., Chowdhury, M.N.R., Rashid, M.A., Chaity, N.I., Zhao, C., Xiao, J., Hafez, E.E., Khan, S.A., & Mohamed, I.N. (2022). Antidiabetic Phytochemicals from Medicinal Plants: Prospective Candidates for New Drug Discovery and Development. Front. Endocrinol.,13, 800714. https://doi.org/10.3389/fendo.2022.800714
Antony, J. R., Saroj, B., Nazir, A.P., Gopal, S., Vineeta, M.K., Chakravarty, S., & Bussmann, R.W. (2018). Indigenous uses of ethnomedicinal plants among forest-dependent communities of Northern Bengal, India. Journal of Ethnobiology and Ethnomedicine, 14(8), 2-28. https://doi.org/10.1186/s13002-018-0208-9
ASTM, D. (1989). Standards test method for volatile matter in the analysis sample of coal and coke, in: Gaseous Fuels. Coal and Coke, 5, 305.
Bagchi, N., Sharma, A., & Sar, S.K. (2021). Characterization of Few Fabaceous Plants towards their Medicinal Application. Indian Journal of Ecology, 48(6), 1766-1771.
Balamurugan, V., Sheerin, F.M.A., & Sreenithi, V. (2019). A guide to phytochemical analysis, IJARIIE. pp. 5.
Banerjee, J., Biswas, S., Madhu, N.R., Karmakar, S. R., & Biswas. S. J. (2014). A better understanding of pharmacological activities and uses of phytochemicals of Lycopodium clavatum: A review. Journal of Pharmacognosy and Phytochemistry, 3(1), 207-210
Eljounaidi, K., & Lichman, B.R. (2020). Nature's Chemists: The Discovery and Engineering of Phytochemical Biosynthesis. Front. Chem., 8, 596479. http://doi.org/10.3389/fchem.2020.596479
Ezikanyi, D.N., & Lilian, A.C. (2021). Studies on proximate and phytochemical constituents of Euphorbia hirta L. and Mucuna pruriens Seed Var. IOSR Journal of Pharmacy (IOSRPHR), 11(12), 17-22
Iskandar, B., Lukman, A., Syaputra, S., Al-Abrori, U.N.H., Surboyo, M.D.C., & Lee, C.K. (2021). Formulation, characteristics and anti-bacterial effects of Euphorbia hirta L. mouthwash. J. Taibah. Univ. Med. Sci., 17(2), 271-282. https://doi.org/10.1016/j.jtumed.2021.08.009
Jain, V., Dudhal, A., Chitali, R., & Bankar, P. (2021). A Survey of some medicinally important plants of the family Euphorbiaceae from Baramati area of Pune district of Maharashtra, India. International Journal of Botany studies, 6(4), 456-462.
Junaid, R.S., & Patil, M.K., (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603-608. https://doi.org/10.22271/chemi.2020.v8.i2i. 8834
Ma, Y., Dias, M.C., & Freitas, H. (2020). Drought and Salinity Stress Responses and MicrobeInduced Tolerance in Plants. Front. Plant Sci., 11, 591911. https://doi.org/10.3389/fpls.2020.591911
Maiti, A., Madhu, N.R., & Manna, C. K. (2010). Ethnomedicine used by the tribal people of the district Purulia, W. B., India in controlling fertility and experimental study. Pharmacologyonline, 1, 783-802.
Maiti, A., Madhu, N.R., & Manna, C. K. (2013). Natural products traditionally used by the tribal people of the Purulia district, West Bengal, India for the abortifacient purpose. International Journal of Genuine Medicine, 3(2), e14:1-4.
Mondal, R., Arvind, N., & Manish, M. (2021). Acalypha indica – a boon to mankind. World Journal of Pharmaceutical Research, 10(3), 764-798. https://doi.org/10.20959/wjpr20213-19890
Nazril, N., Hazali, H., Ibrahim, M., Masri, M. & Ayob, M. (2016). Preliminary studies on Acalypha indica: Proximate analysis and phytochemical screening. International Journal of Pharmacy and Pharmaceutical Sciences, 8(3), 406-408.
Ogbuagu, E., Ogbuagu, U., Prince, C.U., Ifeoma, N.N., & Augustine, I.A. (2020). Qualitative Determination of the Phytochemical Composition of Ethanolic Extract of Xylopia aethiopica Fruit. Asian Journal of Medical Principles and Clinical Practice, 3(4), 45-52.
Roy, R., Chakraborty, A., Jana, K., Sarkar, B., Biswas, P., & Madhu, N.R. (2023). The Broader Aspects of Treating Diabetes with the Application of Nanobiotechnology. Springer Nature Singapore Pte Ltd., pp. 137–162. https://doi.org/10.1007/978-981-19-0027-3_7
Sahira, B.K., & Cathrine, L. (2015). General Techniques Involved in Phytochemical Analysis. International Journal of Advanced Research in Chemical Science (IJARCS), 2(4), 25-32.
Sarkar, B., & Madhu, N. R. (2017). Knowledge of traditional medicines and practices used for reproductive health problems. Review article. UGC-sponsored National Seminar on “Medicinal Plants, Health and Environment. (Edn. 1) Nectar Publisher, Kolkata. pp. 45-47.
Sarkar, B., Bhattacharya, P., Chen, C.Y., Maity, J.P., & Biswas, T. (2022). A comprehensive characterization and therapeutic properties in ripened Noni fruits (Morinda citrifolia L.). Int. J. of Exp. Research and Review, 29, 10-32. https://doi.org/10.52756/ijerr.2022.v29.002
Sarkar, B., Biswas, P., Acharya, C.K., Ghorai, S.K., Nahar, N., Jana, S.K., Ghosh, S., Sarkar, D., Behera, B., & Madhu, N.R. (2021). Knowledge of Traditional Indian Medicinal Plants for the Management of COPD. Chettinad Health City Medical Journal, 10(4), 184-189.
Sarkar, S., Sadhu, S., Roy, R., Tarafdar, S., Mukherjee, N., Sil, M., Goswami, A., Madhu, N.R. (2023). Contemporary Drifts in Diabetes Management. Int. J. App. Pharm., 15( 2), 1-9.
Silva, G.O., Abeysundara, A.T., & Aponso, M.M. (2017). Extraction methods, qualitative and quantitative techniques for screening of phytochemicals from plants. American Journal of Essential Oils and Natural Products, 5(2), 29-32.
Singh, V., & Kumar, R. (2017), Study of Phytochemical Analysis and Antioxidant Activity of Allium sativum of Bundelkhand Region. International Journal of Life Sciences Scientific Research, 3(6),1451- 1458. https://doi.org/10.21276/ijlssr.2017.3.6.4
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