Assessment of Micronucleation and Abnormal Nucleation in the Peripheral Erythrocytes of the Fish Mystus gulio of Hooghly River Downstream as per Seasonal Variation
DOI:
https://doi.org/10.52756/ijerr.2023.v36.029Keywords:
Genotoxicity, heavy metals, Hooghly River, micronucleus, Mystus gulio, nuclear abnormalitiesAbstract
The aim of the study was to identify the frequencies of micro nucleations (MN) and nuclear abnormalities (NAs) in the peripheral erythrocytes of fish (Mystus gulio Ham. – Buch.) inhabiting downstream at three locations of river Hooghly, West Bengal, India. The study area mainly consists of three sampling sites, viz., Budge Budge (Bg), Batanagar (Bt) and Birlapur (Br), which were selected. The present study is a first-time endeavour to know the environmental status with particular reference to water pollution through genetic biomonitoring in the Hooghly River of a middle stretch from Batanagar to Birlapur near Diamond-Harbour coastal zone in the inhabiting fish (M. julio). The genotoxic effect, especially induction of MN and NAs in the peripheral erythrocytes, was done on the studied fish. In the case of MN frequencies (%), the values for sites Bt1 & Bt2, Bg1 & Bg2 and Br1 & Br2 were observed 1.92±0.10, 1.40±0.24 and 2.00±0.13, respectively were increased during pre-monsoon season compared to post-monsoon season (1.83 ±0.15, 1.36 ±0.04 and 1.91 ±0.12, respectively). The frequencies (%) of NA, such as lobed nuclei (LN), blebbed nuclei (BLN), notch nuclei (NN), bi-nuclei (BN), dumble-shaped nuclei (DSN), retracted nuclei (RN), nuclear caryolysis (NC), and fragmented nuclei (FN) values, were also observed in the fishes of three study sites. In the case of NA frequencies (%), the values for sites Bt1&Bt2, Bg1&Bg2 and Br1&Br2 were observed higher for BLN (1.54±0.09, 1.14±0.07 and 1.77±0.10, respectively), BN (1.09±0.04, 0.85±0.21 and 1.32±0.06, respectively), NN (1.02±0.09, 0.61±0.16 and 1.18±0.06, respectively), LN (1.86±0.08, 1.22±0.07 and 2.12±0.11, respectively), DSN (2.22±0.22, 1.69±0.08 and 2.56±0.11, respectively), RN (2.33±0.15, 1.82±0.13 and 2.73±0.05, respectively), FN (2.20±0.10, 1.72±0.08 and 2.56±0.13, respectively) and NC (3.01±0.06, 2.72±0.11 and 3.32±0.08, respectively) during pre-monsoon season when compared to post-monsoon season (BLN = 1.50 ±0.07, 1.06 ±0.03 and 1.73 ±0.07; BN = 0.83 ±0.11, 0.62 ±0.11 and 1.18 ±0.06; NN = 0.77 ±0.15, 0.43 ±0.11 and 1.05 ±0.03; LN = 1.42 ±0.06, 1.14 ±0.06 and 2.06 ±0.08; DSN = 1.76 ±0.06, 1.34 ±0.05 and 2.36±0.08; RN = 1.68 ±0.08, 1.30 ±0.06 and 2.31 ±0.08; FN = 1.59 ±0.08, 1.18 ±0.04 and 2.46±0.11 and NC = 2.58 ±0.53, 2.37 ±0.43 and 3.18 ±0.09, respectively). This study provides an important impact of mutagenic risk on the fish specimen, which may vanish due to the long-term effect of genotoxins or a combination of other pollutants. This specimen may show an alarming impact of water pollution, and this study is suggested for future study with other fish species to know the risk of vulnerability of mutation.
References
Abdullah, S., Naz, H., Abbas, Z., Nazir, U., Basharat, M., Ahmed, T., & Qazi, A.A. (2021). Micronucleus assay as a biomarker to diagnose lead, chromium and cadmium induced genotoxicity in erythrocytes of carnivorous fish, Wallago attu. Punjab Univ J Zool, 36(2), 153-158. https://doi.org/10.17582/journal.pujz/2021.36.2.153.158
Al-Sabti, K., & Metcalfe, C. D. (1995). Fish micronuclei for assessing genotoxicity in water. Mutation Research, 343, 121-135. https://doi.org/10.1016/0165-1218(95)90078-0
Begum, M., Alam, M. J., Islam, M. A., & Pal, H. K. (2008). On the food and feeding habit of an estuarine catfish (Mystus gulio Hamilton) in the south-west coast of Bangladesh. University Journal of Zoology Rajshahi University, 27, 91-94. https://doi.org/10.3329/ujzru.v27i0.1962
Bhattacharya, P., Samal, A., & Bhattacharya, T. (2016). Sequential extraction for the speciation of trace heavy metals in Hoogly river sediments, India. International Journal of Experimental Research and Review, 6, 39-49.
Bonnail, E., Antón-Martín, R., Riba, I., & DelValls, T. A. (2019). Metal distribution and short-time variability in recent sediments from the Ganges river towards the Bay of Bengal (India). Geosciences, 9, 260. https://doi.org/10.3390/geosciences9060260
Brahamet, R. P., Blazer, V. S., Shaw, C. H., & Mazik, P. M. (2017). Micronuclei and other erythrocyte nuclear abnormalities in fishes from the Great Lakes Basin, USA. Environmental and Molecular Mutagenesis, 58(8), 570-581. https://doi.org/10.1002/em.22123
Chakraborty, U., Talapatra, S. N., & Chatterjee, T. K. (2023). Assessment of micronucleation and nuclear abnormalities in peripheral erythrocytes of deformed fish (Heteropneustes fossilis Bloch). International Journal of Science and Research,12(9), 654-658. https://doi.org/10.21275/SR23902140743
Da Silva Souza, T., & Fontanetti, C. S. (2006). Micronucleus test and observation of nuclear alterations in erythrocytes of Nile tilapia exposed to waters affected by refinery effluent. Mutation Research, 605(1-2), 87-93. https://doi.org/10.1016/j.mrgentox.2006.02.010
Elgendy, M. Y., Abumourad, I. K., Mohamad Ali, S. E. M., Soliman, W. S. El-Din, Ibrahim, T. B. El-Din & Abbas, W. T. (2017). Health status and genotoxic effects of metal pollution in Tilapia zillii and Solea vulgaris from polluted aquatic habitats. Interna J of Zoological Research,13(2), 54-63. https://doi.org/10.3923/ijzr.2017.54.63
Fenech, M. (1993). The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutation Research, 285(1), 35-44. https://doi.org/10.1016/0027-5107(93)90049-L
Fenech, M., Chang, W. P., Kirsch-Volders, M., Holland, N., Bonassi, S., Zeiger, E., & Human Micron Nucleus project (2003). HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutation Research, 534(1-2), 65-75. https://doi.org/10.1016/S1383-5718(02)00249-8
Flores-Galván, M. A., Daesslé, L. W., Arellano-García, E., Torres-Bugarín, O., Macías-Zamora, J. V., & Ruiz-Campos, G. (2020). Genotoxicity in fishes environmentally exposed to As, Se, Hg, Pb, Cr and toxaphene in the lower Colorado River basin, at Mexicali valley, Baja California, México. Ecotoxicology (London, England), 29(4), 493-502. https://doi.org/10.1007/s10646-020-02200-9
Goswami, D. N., & Sharda, S. S. (2014). Determination of heavy metals, viz. cadmium, copper, lead and zinc in the different matrices of the Ganges river from Rishikesh to Allahabad through differential pulse anodic striping voltametry. International Journal of Advanced Research in Chemical Science, 1(5), 7-11.
Gupta, A., Rai, D. K., Pandey, R. S., & Sharma, B. (2009). Analysis of some heavy metals in the riverine water, sediments and fish from river Ganges at Allahabad. Environmental Monitoring and Assessment, 157, 449. https://doi.org/10.1007/s10661-008-0547-4
Hussain, B., Sultana, T., Sultana, S., Masoud, M. S., Ahmed, Z., & Mahboob, S. (2018). Fish eco-genotoxicology: Comet and micronucleus assay in fish erythrocytes as in situ biomarker of freshwater pollution. Saudi Journal of Biological Sciences, 25(2), 393-398. https://doi.org/10.1016/j.sjbs.2017.11.048
Igbo, J. K., Chukwu, L. O., Oyewo, E. O., Zelikoff, J. T. (2018). Micronucleus assay and heavy metals characterization of e-waste dumpsites in Lagos and Osun States, Southwest Nigeria.Journal of Applied Sciences and Environmental Management, 22(3), 329-337.
Inayat, I., Batool, A. I., Rehman, M. F. U., Ahmad, K. R., Kanwal, M. A., Ali, R., Khalid, R., & Habib, S. S. (2023). Seasonal variation and association of heavy metals in the vital organs of edible fishes from the river Jhelum in Punjab, Pakistan. Biological Trace Element Research, https://doi.org/10.1007/s12011-023-03730-z.
Mandal, M. (2020). Assessment of lead accumulation in muscle and abnormal nucleation in the peripheral erythrocytes of fish (Mystus cavisus HAM. -BUCH.) of Hooghly river downstream. Journal of Advanced Scientific Research, 11(1), 202-207.
Mandal, M., & Chatterjee, T. K. (2022). Study of correlation between downstream stretches as study sites in Hooghly river and lead content in sediment and muscle of fish specimen (Mystus gulio HAM. - BUCH.). Journal of Advanced Scientific Research, 13(05), 151-153.
Mandal, M., & Chatterjee, T. K. (2023). Study of lead bioaccumulation in fish specimen (Mystus gulio HAM. – BUCH.) of Hooghly river downstream as per seasons. International Journal of Science and Research,12(8), 1-3. https://doi.org/10.55218/JASR.202213519
Mauryaa, P. K., Malika, D. S., Yadav, K. K., Kumar, A., Kumar, S., & Kamyabe, H. (2019). Bioaccumulation and potential sources of heavy metal contamination in fish species in river Ganga basin: Possible human health risks evaluation. Toxicology Reports, 6, 472-481. https://doi.org/10.1016/j.toxrep.2019.05.012
Mitra, A. (1998). Status of coastal pollution in West Bengal with special reference to heavy metals. J Indian Ocean Studies, 5(2), 135-138.
Mitra, A., Chowdhury, R., & Banerjee, K. (2012). Concentrations of some heavy metals in commercially important finfish and shellfish of the river Ganga. Environmental Monitoring and Assessment, 184, 2219-2230. https://doi.org/10.1007/s10661-011-2111-x
Mondal, B., Bhattarcharya, K., Swarnakar, S., & Talapatra, S. N. (2021). Assessment of nuclear abnormalities in the peripheral erythrocytes of fish specimen of Sundarbans coastal zone, West Bengal, India. Pollution Research, 40(4), 1284-1288.
Mondal, P., Adhikary, P., Sadhu, S., Choudhary, D., Thakur, D., Shadab, M., Mukherjee, D., Parvez, S., Pradhan, S., Kuntia, M., Manna, U., & Das, A. (2022). Assessment of the impact of the different point sources of pollutants on the river water quality and the evaluation of bioaccumulation of heavy metals into the fish ecosystem thereof. International Journal of Experimental Research and Review, 27, 32-38. https://doi.org/10.52756/ijerr.2022.v27.003
Nagpure, N. S., Srivastava, R., Kumar, R., Dabas, A., Kushwaha, B., & Kumar, P. (2015). Assessment of pollution of river Ganges by tannery effluents using genotoxicity biomarkers in murrel fish, Channa punctatus (Bloch). Indian Journal of Experimental Biology,53, 476-483.
Nagpure, N. S., Srivastava, R., Kumar, R., Dabas, A., Kushwaha, B., & Kumar, P. (2016). Mutagenic, genotoxic and bioaccumulative potentials of tannery effluents in freshwater fishes of river Ganga.Human and Ecological Risk Assessment: An International Journal, 23(1), 98-111. https://doi.org/10.1080/10807039.2016.1229116
Omar, W. A., Zaghloulb, K. H., Abdel-Khaleka, A. A., & Abo-Hegaba, S. (2012). Genotoxic effects of metal pollution in two fish species, Oreochromis niloticus and Mugil cephalus, from highly degraded aquatic habitats. Mutation Research, 746, 7-14. https://doi.org/10.1016/j.mrgentox.2012.01.013
Palhares, D., & Grisolia, C. K. (2002). Comparison between the micronucleus frequencies of kidney and gill erythrocytes in tilapia fish, following mitomycin C treatment. Genetics and Molecular Biology, 25(3), 281-284. https://doi.org/10.1590/S1415-47572002000300005
Paul, D. (2017). Research on heavy metal pollution of river Ganga: A review. Annals of Agrarian Science, 15, 278-286. https://doi.org/10.1016/j.aasci.2017.04.001
Roy, J., Samal, A., Maity, J., Bhattacharya, P., Mallick, A., & Santra, S. (2022). Distribution of heavy metals in the sediments of Hooghly, Jalangi and Churni river in the regions of Murshidabad and Nadia districts of West Bengal, India. International Journal of Experimental Research and Review, 27, 59-68. https://doi.org/10.52756/ijerr.2022.v27.007
Sarkar, S. K., Mondal, P., Biswas, J. K., Kwon, E. E., Ok, Y. S., & Rinklebe, J. (2017). Trace elements in surface sediments of the Hooghly (Ganges) estuary: distribution and contamination risk assessment. Environmental Geochemistry and Health, 39, 1245-1258. https://doi.org/10.1007/s10653-017-9952-3
Talapatra, S. N., & Banerjee, S. K. (2007). Detection of micronucleus and abnormal nucleus in erythrocytes from the gill and kidney of Labeo bata cultivated in sewage-fed fish farms. Food and Chemical Toxicology: an international journal published for the British Industrial Biological Research Association, 45(2), 210-215. https://doi.org/10.1016/j.fct.2006.07.022
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