Study of Glucose-6 Phosphatase activity in Clarias batrachus (Linn.) after feeding the probiotic fish feed

Anusaya Mallick

doi:10.52756/ijerr.2017.v12.002

Anusaya Mallick Department of Environmental Science, University of Kalyani, Kalyani-741235, West Bengal,

DOI: https://doi.org/10.52756/ijerr.2017.v12.002

Keywords: Clarias batrachus, glucose-6 phosphatase, probiotic, lactic acid bacteria (LAB)

Abstract

Glucose-6 Phosphatase activity was studied in the air breathing fresh water catfish (Clarias batrachus) by giving the probiotic feed. The experiment was conducted in laboratory condition with glass jar aquarium. Bacteriological and different biochemical tests like cytochrome oxidase, fermentative and catalase test were analysed along with certain physiological parameters of water samples. Clarias batrachus was fed by prepared four diets, F1, F2, F3 and F4. The diets were prepared using fishmeal, soyabean meal, groundnut oil cake, rice bran, vitamin mineral mixture and different concentration of probiotics. The Clarias batrachus in experimental treatments were fed by the prepared diets upto six weeks. The significant amount of Lactic acid bacteria (LAB) was found in the gut microflora of the experimental fish. Glucose 6- phophatase activity was decreased in liver with increase in probiotic concentration in feed. But the enzyme activity in kidney, gill and stomach did not follow any trend. The water quality parameters like pH, CO2, DO, alkalinity, ammonia concentrations of the experimental tanks were varied significantly with treatment time.

References

Abareethan, M. and Amsath, A. (2015). Characterization and evaluation of probiotic fish feed. International Journal of Pure and Applied Zoology. 3 (2): 148-153.

Abraham, V. A., Faleiro, J. R., Al-Shuaibi, M. A. and Prem Kumar, T. (2000). A strategy to manage red palm weevil Rhynchophorus ferrugineus Oliv. on date palm Phoenix dactylifera L. Its successful implementation in Al-Hassa, Kingdom of Saudi Arabia. Pestology. 24(12): 23-30.

Alikunhi, K. H. (1957). Fish culture in India. Farm Bulletin. (20): 1-144.

APHA, (1998). Standard methods for the examination of water and wastewater, 16th ed., American Public Health Association Water Pollution Central Federation and American Water Works Association: New York, USA.

Åsgård, T. and Austreng, E. (1995). Optimal utilization of marine proteins and lipids for human interest. Sustainable fish farming. Pp.79- 87.

Askarian, F., Zhou, Z., Olsen, R. E., Sperstad, S., Ringø, E. (2012). Culturable autochthonous gut bacteria in Atlantic salmon (Salmo salar L.) fed diets with or without chitin. Characterization by 16S rRNA gene sequencing, ability to produce enzymes and in vitro growth inhibition of four pathogens, Aquaculture. 1-8: 326-329.

Balazs, R., Patel, A. J. and Richter, D. (1973). Metabolic compartments in the brain: their properties and relation to morphological structures. In Metabolic Compartmentation in the Brain. Pp. 167-184.

Chakrabarti, R., Mansingh Rathore, R., Mittal, P. and Kumar, S. (2006). Functional changes in digestive enzymes and characterization of proteases of silver carp (male) and bighead carp (female) hybrid, during early ontogeny. Aquaculture. 253(1–4): 694–702.

Fuller, M. F., McWilliam, R., Wang, T. C. and Giles, L. R. (1989). The optimum dietary amino acid pattern for growing pigs. British Journal of Nutrition. 62(2): 255-267.

Hastings, J. W. and Mitchell, G. (1971). Endosymbiotic bioluminescent bacteria from the light organ of pony fish. The Biological Bulletin. 141(2): 261-268.

Hastings, S. P. (1976). On the existence of homoclinic and periodic orbits for the Fitz Hugh-Nagumo equations. The Quarterly Journal of Mathematics. 27(1): 123-134.

Hastings, A. (1977). Spatial heterogeneity and the stability of predator-prey systems. Theoretical population biology. 12(1): 37-48.

Lipton, A. P. (1998). Disease management in shrimp culture with species referenece to probiotic and additives of workshop on National Aquaculture Week. January February 1997. Aquaculture Foundation of India, Chennai: 20-219.

Macmillan Education UK. FAO. (1998). The state of the world’s plant genetic resources for food and agriculture. Food and Agriculture Organization of the United Nations, Rome, Italy. Pp. 510.

Mishra, S. K. and Hermsmeyer, K. (1994). Selective inhibition of T-type Ca2+ channels by Ro 40- 5967. Circulation research. 75(1): 144-148.

Mohapatra, S., Chakraborty, T., Prusty, A. K., Das, P., Pani Prasad, K. and Mohanta, K. N. (2012). Use of different microbial probiotics in the diet of rohu, Labeo rohita fingerlings: effects on growth, nutrient digestibility and retention, digestive enzyme activities and intestinal microflora. Aquaculture Nutr. 18(1): 1-11.

Murthy, H. S. and Naik, A. T. R. (2002). Growth performance of Cirrhinus mrigala in relation to a natural probiotic feed supplement, Biovet-YC. Fish. Chimes. 22: 54-56.

Olsen, S. F., Sorensen, J. D., Secher, N. J., Hedegaard, M., Henriksen, T. B., Hansen, H. S. and Grant, A. (1992). Randomised controlled trial of effect of fish-oil supplementation on pregnancy duration. The Lancet. 339(8800): 1003-1007.

Panigrahi, A. and Azad, I.S. (2007). Microbial intervention for better fish health in aquaculture: the Indian scenario. Fish Physiol. Biochem. 33: 429-440.

Plummer, D. T. (1988). An Introduction to practical biochemistry. 3rd edition, Tata McGraw-Hill Education.

Prabhu, R., Colfer, C. J. P. and Dudley, R. G. (1999). Guidelines for developing, testing and selecting criteria and indicators for sustainable forest management: a C&I developer's reference. (1): CIFOR. Ringø, E, and Gatesoupe, F. J (1998). Lactic acid bacteria in fish. Aquaculture. 160: 177-203.

Saunders, A. D., Tarney, J. and Weaver, S. D. (1980). Transverse geochemical variations across the Antarctic Peninsula: implications for the genesis of calc-alkaline magmas. Earth and Planetary Science Letters. 46(3): 344-360.

Sharma, J. G., Mittal, P. and Chakrabarti, R. (2008). Development of survivorship model for UV-B irradiated Catla catla larvae. Aquatic Ecology. 42(1): 17–23.

Sharpe, W. F. (1964). Capital asset prices: A theory of market equilibrium under conditions of risk. The Journal of finance. 19(3): 425-442.

Taoka, Y., Maeda, H., Jo, J.Y., Jeon, M. N., Bai, S. C., W.J. Lee, K. Yuge, Koshio, S. (2006). Growth, stress tolerance and non-specific immune response of Japanese flounder, Paralichthys olivaceus to probiotics in a closed recirculating system. Fisheries Sci. 72: 310- 321.

Viola, S. and Arieli, Y. (1987). Non hormonal growth promoters for tilapia and carp. 1. Screening-tests in cages. Bamidgeh. 39(2): 31- 38.