Secondary Metabolite Fingerprinting and Antioxidant Potency of Eleusine indica: An Integrated Chromatography and In silico Investigation
Abstract
Natural regimens and constituents serve as the primary sources for drug conservation, and each herbal regimen possesses unique ethnomedicinal and biological properties. Therefore, researchers are currently more interested in exploring the biological activity of existing plant species with the most systematic approach to be used in the specific therapeutics proposed, either crude extracts or bioactive metabolites. The present study also explores the antioxidant potency of ethanolic extracts of Eleusine indica (a weed in an agricultural field) through chemical fingerprinting analyses through high-performance thin-layer chromatography (HPTLC), followed by gas chromatography-mass spectrometry (GCMS) and in silico investigation in a systematic way. Briefly, this investigation aims to observe the presence of secondary metabolites associated with antioxidant activity. After extraction, a fingerprint study was carried out with the mobile phase consisting of ethyl acetate, acetic acid, formic acid, and water in the ratio 10:1.1:1.1:2.3 by volume, where more than eight fluorescent and non-fluorescent bands were found, with the highest density peak area at Rf 0.87 and the lowest at Rf 0.18. As per the GCMS report ethanolic extracts of E. indica are composed of several metabolites. Further, based on the higher Rf value, we have selected seven (EI_1 to EI_7) that constitute molecular docking against three target enzymes: cyclooxygenase-2 (PDB ID: 5F1A), peroxiredoxin-5 (PDB ID: 1HD2), and haemoxygenase 1 (PDB ID: 3CZY) to assess the antioxidant potency. Overall, the results revealed that EI_7 (2,7-dioxatricyclo [4.4. 0.0(3,8)] decane-4,5-diol) showed similar potency with ideal drug-ability profiles similar to ascorbic acid and was considered a lead for further therapeutic use as a complementary agent.
References
Abioye, V.F., Babarinde, G.O., Ogunlakin, G.O., Adejuyitan, J.A., Olatunde, S.J., & Abioye, A.O. (2022). Varietal and processing influence on nutritional and phytochemical properties of finger millet: A review. Heliyon, 8(12), e12310. https://doi.org/10.1016/j.heliyon.2022.e12310.
Al-Zubairi, A.S., Abdul, A.B., Abdelwahab, S.I., Peng, C.Y., Mohan, S., & Elhassan, M.M. (2011). Eleucine indica Possesses Antioxidant, Antibacterial and Cytotoxic Properties. Evid Based Complement Alternat Med., 2011, 965370. https://doi.org/10.1093/ecam/nep091.
Attimarad, M., Ahmed, K.K., Aldhubaib, B.E., & Harsha, S. (2011). High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery. Pharm Methods, 2(2), 71-5. https://doi.org/10.4103/2229-4708.84436.
Banerjee, J., Biswas, S., Madhu, N.R., Karmakar, S. R. and Biswas. S. J. (2014). A better understanding of pharmacological activities and uses of phytochemicals of Lycopodium clavatum: A review. Journal of Pharmacognosy and Phytochemistry, 3(1), 207-210
Bārzdiņa, A., Paulausks, A., Bandere, D., & Brangule, A. (2022). The Potential Use of Herbal Fingerprints by Means of HPLC and TLC for Characterization and Identification of Herbal Extracts and the Distinction of Latvian Native Medicinal Plants. Molecules, 27(8), 2555. https://doi.org/10.3390/molecules27082555.
Bhatta, K., Samal, H., & Mohanty, P. (2023). Isozyme profiling of Antioxidant Enzyme in Macrotyloma uniflorum. Int. J. Exp. Res. Rev., 36, 156-165. https://doi.org/10.52756/ijerr.2023.v36.015a
Elmore, M.T., Diehl, K.H., Di, R., Chen, J., Patterson, E.L., Brosnan, J.T., Trigiano, R.N., Tuck, D.P., Boggess, S.L., & McDonald, S. (2022). Identification of two Eleusine indica (goosegrass) biotypes of cool-season turfgrass resistant to dithiopyr. Pest Manag Sci., 78(2), 499-505. https://doi.org/10.1002/ps.6654.
Gonzaga, E.A., Taño, T.V., & Gonzaga, L.J.A. (2023) In vivo anthelmintic activity of Eleusine indica extracts against gastrointestinal nematodes of goats. J. Adv. Vet. Anim. Res., 10(3), 342-346. https://doi.org/10.5455/javar.2023.j686.
Hijam, A., Koijam, A., & Haobam, R. (2024). Methanol extract of Isodon ternifolius (D. Don) KUDO leaves has antimicrobial and antioxidant activities but no neuroprotective activity. International Journal of Experimental Research and Review, 39(Spl Volume), 200-212. https://doi.org/10.52756/ijerr.2024.v39spl.016
Hore, P., & Bhaben, T. (2023). Toxic Effect of 2,4-D on Cytology of Vigna radiata (L.) Wilczek. Int. J. Exp. Res. Rev., 30, 276-81. https://doi.org/10.52756/ijerr.2023.v30.025.
Iqbal, M., & Gnanaraj, C. (2012). Eleusine indica L. possesses antioxidant activity and precludes carbon tetrachloride (CCl₄)-mediated oxidative hepatic damage in rats. Environ Health Prev Med., 17(4), 307-15. https://doi.org/10.1007/s12199-011-0255-5.
Jakkana, V., & Yamala, S. (2024). Isolation, Identification and in-Silico Characterization of Bioactive Peptide from the Venom Sac of Conus inscriptus. International Journal of Experimental Research and Review, 38, 1-14. https://doi.org/10.52756/ijerr.2024.v38.001
Koli, R., Mannur, V.S., & Shetti, P.P (2024) Robust high-performance thin-layer chromatography (HPTLC) method for stability assessment and simultaneous quantification of epigallocatechin-3-gallate and rosmarinic acid in lipid-based nanoparticles and biological matrices. Phytochem Anal. https://doi.org/10.1002/pca.3360.
Konappa, N., Udayashankar, A.C., Krishnamurthy, S., Pradeep, C.K., Chowdappa, S., & Jogaiah, S. (2020) GC-MS analysis of phytoconstituents from Amomum nilgiricum and molecular docking interactions of bioactive serverogenin acetate with target proteins. Sci. Rep., 10(1), 16438. https://doi.org/10.1038/s41598-020-73442-0.
Kowalska, T., & Sajewicz M. (2022). Thin-Layer Chromatography (TLC) in the screening of botanicals-its versatile potential and selected applications. Molecules, 27(19), 6607. https://doi.org/10.3390/molecules27196607.
Kumar, A., Metwal, M., Kaur, S., Gupta, A.K., Puranik, S., Singh, S., Singh, M., Gupta, S., Babu, B.K., Sood, S., & Yadav, R. (2016). Nutraceutical Value of Finger Millet [Eleusine coracana (L.) Gaertn.], and Their Improvement Using Omics Approaches. Front Plant Sci., 7, 934. https://doi.org/10.3389/fpls.2016.00934.
Li, F., Wu, H., Sun, L.L., Wu, H., Wang. R., Li, S.P., Wang, W.Y., Dai, L., Zhang, Z.R., Fu, J., & Deng, R. (2018) Quantitative Analysis of Multi-components by Single Marker and Fingerprint Analysis of Achyranthes bidentata Blume. J. Chromatogr. Sci., 56(7), 595-603. https://doi.org/10.1093/chromsci/bmy031.
Li, J., Zhang, Z., Lei, Q., Lu, B., Jin, C., Liu, X., Wang, Y., & Bai, L. (2022). Multiple herbicide resistance in Eleusine indica from sugarcane fields in China. Pestic. Biochem. Physiol., 182, 105040. https://doi.org/10.1016/j.pestbp.2022.105040.
Magadelin, B.J., Sinthuja, S.A., & Ahamed, S.J. (2023). Phytochemical Screening and Biological activities of Eleusine indica leaf extract. Biological Forum – An Int. J., 15(3), 444-449.
Nakkala, K., & Laddha, K.S. (2022). Development of a validated high-performance thin-layer chromatography method for quantification of lupeol from different parts of Bauhinia acuminata. Biomed Chromatogr., 36(10), e5448. https://doi.org/10.1002/bmc.5448.
Ong, S.L., Nalamolu, K.R., & Lai, H.Y. (2017). Potential lipid-lowering effects of Eleusine indica (L) Gaertn. extract on high-fat-diet-induced hyperlipidemic rats. Pharmacogn Mag., 13(1), S1-S9.
Pandey, G., Khatoon, S., Pandey, M.M., & Rawat, A.K.S. (2018). Altitudinal variation of berberine, total phenolics and flavonoid content in Thalictrum foliolosum and their correlation with antimicrobial and antioxidant activities. J. Ayurveda Integr. Med., 9(3), 169-176. https://doi.org/10.1016/j.jaim.2017.02.010.
Rami, N., Kulkarni, B., Chibber, S., Jhala, D., Parmar, N., & Trivedi, K. (2023). In vitro antioxidant and anticancer potential of Annona squamosa L. Extracts against breast cancer. Int. J. Exp. Res. Rev., 30, 264-275. https://doi.org/10.52756/ijerr.2023.v30.024
Sahoo, A., Fuloria, S., Swain, S.S., Panda, S.K., Sekar, M., Subramaniyan, V., Panda, M., Jena, A.K., Sathasivam, K.V., & Fuloria, N.K (2021b). Potential of marine terpenoids against SARS-COV-2: An in-silico drug development approach. Biomedicines, 9(11), 1505. https://doi.org/10.3390/biomedicines9111505.
Sahoo, A., Swain, S.S., Paital, B., & Panda, M. (2022a). Combinatorial approach of vitamin C derivative and anti-HIV drug-darunavir against SARS-CoV-2. Front Biosci. (Landmark Ed), 27(1), 10. https://doi.org/10.31083/j.fbl2701010.
Sahu, T.K., Sahoo, N., Pattnaik, G., Samal, H.B., Behera, A., & Kar, B. (2024). Alcoholic Extracts of Eleusine indica as Alternative Diuretic Regimens: A Computational Based Investigation. Int. J. Exp. Res. Rev., 37(Spl.), 11-21. https://doi.org/10.52756/ijerr.2024.v37spl.002
Saidi, N., Kadir, J., & Hong, L.W. (2016). Genetic diversity and morphological variations of goosegrass [Eleusine indica (L.) Gaertn] ecotypes in Malaysia. Weed Turf. Sci., 5(3), 144-154 http://dx.doi.org/10.5660/WTS.2016.5.3.144
Sarkar, B., Kotal, H.N., Giri, C.K., Mandal, A., Hudait, N., Madhu, N.R., Saha, S., Basak, S.K., Sengupta, J., & Ray, K. (2024). Detection of a bibenzyl core scaffold in 28 common mangrove and associate species of the Indian Sundarbans: potential signature molecule for mangrove salinity stress acclimation. Front. Plant Sci., 14, 1291805. https://doi.org/10.3389/fpls.2023.1291805
Sukor, N.S.M., Zakri, Z.H.M., Rasol, N.E., & Salim, F. (2023). Annotation and Identification of Phytochemicals from Eleusine indica Using High-Performance Liquid Chromatography Tandem Mass Spectrometry: Databases-driven approach. Molecules., 28(7), 3111. https://doi.org/10.3390/molecules28073111.
Swain, S.S., Hussain, T., & Pati, S. (2021). Drug-lead Anti-tuberculosis phytochemicals: A systematic review. Curr. Top Med. Chem., 21(20), 1832-1868. https://doi.org/10.2174/1568026621666210705170510.
Swain, S.S., Singh, S.R., Sahoo, A., Hussain, T., & Pati, S. (2022a). Anti-HIV-drug and phyto-flavonoid combination against SARS-CoV-2: a molecular docking-simulation base assessment. J. Biomol. Struct. Dyn., 40(14), 6463-6476. https://doi.org/10.1080/07391102.2021.1885495
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