Role of 4-Phenylbutyric Acid in DNA and Protein Binding and its Functional Analysis

Dikshita Deka; Alakesh Das; Ashiq Shibili P; Antara Banerjee

doi:10.52756/ijerr.2024.v45spl.017

Authors

Dikshita Deka Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Chennai 603103, India https://orcid.org/0000-0001-5087-9023
Alakesh Das Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Chennai 603103, India https://orcid.org/0000-0003-2595-9309
Ashiq Shibili P Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Chennai 603103, India
Antara Banerjee Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Chennai 603103, India https://orcid.org/0000-0002-5519-6878

DOI:

https://doi.org/10.52756/ijerr.2024.v45spl.017

Keywords:

Anti-bacterial activity, Cytotoxicity, DNA-Binding, 4-Phenylbutyric Acid

Abstract

4-Phenylbutyric acid (4-PBA) is a small molecule known for its protein folding capacity to reduce endoplasmic reticulum (ER)-stress. This study aimed to explore the potential of 4-PBA by studying its interactions with DNA and protein and examining its effects on cellular toxicity and antibacterial activity. UV-VIS absorption spectroscopy demonstrated that 4-PBA effectively binds to calf thymus DNA (CT-DNA), as indicated by an evident hyperchromic shift, suggesting stable intercalating interactions. Similarly, the fluorescence quenching assay demonstrated that 4-PBA also interacts with bovine serum albumin (BSA), reducing fluorescence intensity by occupying specific binding sites on the protein. The cytotoxicity analysis using cell counting kit-8 further showed no significant reduction in cell viability of normal human lung epithelial cell line (L132). Subsequently, 4-PBA also exhibited minimal growth inhibition of Escherichia coli and Staphylococcus aureus bacterial strains, indicating limited antibacterial activity under the tested conditions. Additionally, this study provides a basis for future research towards the molecular mechanisms and therapeutic applications of 4-PBA.

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