Cytotoxic and genotoxic effects of a common food additive monosodium glutamate in mice: a preliminary report
Keywords:
Cytotoxicity, CA, genotoxicity, MI, MSG, SHA
Abstract
In our present study we investigated if the Monosodium glutamate (MSG) has any cytotoxic and genotoxic effect in animal system by studying cytogenetical parameters, namely chromosome aberration (CA), mitotic indices (MI) and sperm head anomaly (SHA) and a histo-pathological parameter i.e., tissue weight / body weight ratio in mice. Experiments were carried out at three different fixation intervals, namely, 7D, 14D and 21D, maintaining suitable controls. MSG was administered to mice at the dose 2 mg / gm of body weight. The investigation reveals that feeding of MSG induced geno-toxicity and cyto-toxicity. These produced greater amount of CA, SHA along with a general increase in the MI at significant levels. Thus, the increased level of genotoxicity and cytotoxicity could be attributed due to chronic feeding of the food additive MSG.
References
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Biswas, S. J., Pathak, S. and Khuda-Bukhsh, A. R. (2004). Assessment of the genotoxic and cytotoxic potential of an anti-epileptic drug, phenobarbital, in mice: a time course study. Mutat Res. 563: 1.
Biswas, S. J. and Khuda-Bukhsh, A. R. (2005). Cytotoxic and genotoxic effects of the azo-dye p-dimethylaminoazobenzene in mice: A time-course study. Mutat Res. 587: 1.
Bresgen, N., Karlhuber, G., Krizbi, I., Bauer, H., Bauer, H. C. and Eckl, P. M. (2003). Oxidative stress in cultured endothelial cells induces choromosomal aberrations, micronuclei and apoptosis. J. Neurosci. Res. 72(3): 327.
Bunyan, J., Murrell, E. A. and Shah, P. P. (1976). The induction of obesity in rodents by means of monosodium glutamate. British J. Nutr. 35: 25.
Das, R. S. and Ghosh, S. K. (2010). Long term effects of monosodium glutamate on spermatogenesis following neonatal exposure in albino mice – a histological study. Nepal Med. Coll. J. 12(3:) 149.
Fisher, R. A. and Yates, F. (1953). Statistical tables for Biological, Agricultural and Medical Research. 4th Edition. Oliver and Boyd, Edinburgh.
Fuke, S. and Shimizu, T. (1993). Sensory and preference aspects of Umami. Trends in Food Sci & Technol. 4: 246.
Garattiini, S. (2000). Glutamic Acid, Twenty Years Later. J. Nutr. 130: 9018.
Harman, D. (1993). Free radical theory of aging:A hypothesis on pathogenesis of senile dementia of the Alzheimer’s type. AGE. 16(1): 23.
Mallick, A., Mohapatra, B. C. and Sarangi, N. (2016). Acute toxicity of sodium chloride on different developmental stages (Egg, Spawn, Fry and Fingerlings) of Labeo rohita (Rohu). International Journal of Experimental Research and Review. 5: 49-57.
Olney, J. W. (1969). Brain lesions, obesity and other disturbances in mice treated with monosodium glutamate. Sci. 164: 719.
Schaumburg, H. H., Byck, R., Gerstl, R. and Mashman, J. H. (1969). Monosodium L-Glutamate: Its pharmacology and role in Chinese restaurant syndrome. Sci. 163: 826.
Schmid, W. (1976). In: Hollaender, A, E. (eds). Chemical Mutagens: Principles and Methods of Detection. Plenum Press, New York.
Singh, K. and Ahluwalia, P. (2003). Studies on the effect of Monosodium Glutamate (MSG) administration on some antioxidant enzymes in the arterial tissue of adult male mice. J Nutr Sci Vitaminol (Tokyo). 49: 145.
Wyrobek, A. J., Watchmaker, G. and Gordon, L. (1984). In: Kilbey, B. J., Legator, M., Nichols, W. and Ramel, C. (eds). Handbook of mutagenecity testing protocols. Elsevier Science, Netherland. Pp. 733.
Yamaguchi, S. (1987). Fundamental properties of umami in human taste sensation. In: Kawamura, Y. and Kare, M.R. (eds). Umami: A Basic Taste. New York: Marcel Dekker. Pp. 41.
Published
2016-12-30
How to Cite
Pramanik, S., & Karmakar, S. (2016). Cytotoxic and genotoxic effects of a common food additive monosodium glutamate in mice: a preliminary report. International Journal of Experimental Research and Review, 8, 98-103. Retrieved from https://qtanalytics.in/journals/index.php/IJERR/article/view/1317
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