Study on the toxicity of neem (Azadirachta indica A. Juss) leaf extracts as phytopiscicide on three life stages of Mozambique tilapia (Oreochromis mossambicus Peters) with special reference to their ethological responses

Kishore Dhara; Surjyo Jyoti Biswas; Susanta Roy Karmakar

Authors

Kishore Dhara Office of the Deputy Direcetor of Fisheries (Gour Banga Zone), Meen Bhaban, Mangalbari, Malda -732 142, West Bengal, India
Surjyo Jyoti Biswas Department of Zoology, Sidho Kanho Birsha University, Purulia-723 104, India
Susanta Roy Karmakar Post Graduate Department of Zoology, Maulana Azad College, Kolkata-700 013, India

Keywords:

Acute toxicity, ethological responses, neem, Oreochromis mossambicus

Abstract

Acute toxicity of leaf extracts of neem (Azadirachta indica A. Juss) on three different stages of fresh water weed fish Oreochromis mossambicus was investigated in the present study. The 24, 48, 72 and 96 h LC50 values for O. mossambicus fry were 3.29, 2.62, 2.19 and 1.67 g/l respectively. These values were 4.96, 3.56, 2.74, 2.27 g/l and 7.58, 7.00, 6.28, 5.83 g/l for juvenile and adult fish respectively. Toxicity factor values of the toxicant to the fish irrespective of stage were increased with the time of exposure. During the study, ethological changes in the adult fish exposed to two sub-lethal concentrations of the toxicant (10 and 20% of 96h LC50) were also observed. It showed an alteration in behaviour pattern with the increasing concentration of the neem leaf extracts and time of exposure.

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